Let’s Dive into VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb
In the fast-paced world of molecular biology, precision and efficiency are paramount. The VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb promises just that: a reliable tool to enhance your electrophoresis experiments. Manufactured by VWR, this comb is designed to work seamlessly with their Midi Plus Horizontal Electrophoresis Systems, known for their versatility and ease of use. The 1.5 mm thickness and 14-tooth design suggest a balance between sample volume and resolution, making it an attractive option for researchers working with various DNA or protein samples.
As a seasoned lab professional, I’ve always sought tools that streamline my workflow. My research frequently involves analyzing PCR products and restriction fragments, requiring consistent and high-quality electrophoresis results. I needed a comb that could handle a decent number of samples per gel while maintaining good band separation.
Upon unboxing, the VWR comb felt robust, constructed from a durable-looking material. The teeth were uniformly spaced and appeared well-formed, suggesting precision in manufacturing. It felt significantly sturdier than some of the cheaper combs I’ve encountered.
I considered other combs from Bio-Rad and Thermo Fisher Scientific, but opted for the VWR comb because it was specifically designed for the VWR Midi Plus system already in my lab. I was excited to see if this comb would improve the resolution and throughput of my electrophoresis runs.
Real-World Testing: Putting VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb to the Test
First Use Experience
My initial test involved separating PCR products of varying sizes (200 bp to 1 kb). I used the VWR Midi Plus Horizontal Electrophoresis System with the VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb to create the wells. The comb fit perfectly into the gel tray, and the wells formed were clean and well-defined.
The 14-tooth design allowed me to load a significant number of samples in a single run, greatly speeding up my process. After the electrophoresis run, the bands were sharp and well-separated, making analysis straightforward.
Extended Use & Reliability
After several weeks of consistent use, the VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb has proven to be incredibly reliable. There are no signs of wear and tear, even after repeated cleaning and use.
Cleaning is simple; a quick rinse with distilled water removes any residual agarose or buffer. Compared to previous combs I’ve used, this one consistently produces cleaner, more uniform wells.
Breaking Down the Features of VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb
Specifications
Description: The VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb creates 14 wells on an agarose gel. The 1.5 mm thickness provides sufficient volume for loading larger samples.
Dimensions: The comb is designed to fit precisely within the VWR Midi Plus Horizontal Electrophoresis System, with overall system dimensions of 17.5W x 26.5L x 9H cm (67/8 x 107/16 x 317/32“). These dimensions ensure compatibility with the gel tray and buffer tank.
Buffer Volume: The system accommodates a buffer volume of 500 mL. A sufficient buffer volume ensures stable pH and temperature during electrophoresis.
Performance & Functionality
The VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb excels in creating consistent, well-defined wells. These wells are essential for accurate sample loading and result in sharper band resolution.
The comb’s 14-tooth design significantly boosts throughput without sacrificing band clarity. While the 1.5mm thickness is ideal for larger sample volumes, it may result in slightly wider bands compared to thinner combs.
Design & Ergonomics
The VWR comb features a simple, yet effective design. It is constructed from a smooth, durable plastic that is easy to handle and clean.
The comb is user-friendly with minimal learning curve. It fits snugly into the gel tray and is easy to remove once the gel has solidified.
Durability & Maintenance
The VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb is built to last. Regular cleaning with distilled water and gentle handling will extend its lifespan.
The comb’s simple design means there are no small parts to break or wear out. Its robust construction ensures it can withstand daily lab use.
Accessories and Customization Options
The VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb is designed specifically for the VWR Midi Plus Horizontal Electrophoresis Systems. While not customizable in itself, the system offers various gel trays and comb options for different applications.
It is compatible with standard electrophoresis buffers and DNA/protein stains. No specific VWR branded accessories are required for its use.
Pros and Cons of VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb
Pros
- Creates well-defined, consistent wells for accurate sample loading.
- 14-tooth design increases throughput, saving time and resources.
- Durable construction ensures long-term reliability.
- Easy to clean and maintain, streamlining workflow.
- Compatible with the versatile VWR Midi Plus Horizontal Electrophoresis System.
Cons
- 5 mm thickness may result in slightly wider bands compared to thinner combs.
- Specifically designed for VWR Midi Plus system; limited compatibility with other systems.
Who Should Buy VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb?
This comb is perfect for molecular biologists and lab technicians who routinely perform agarose gel electrophoresis. It’s especially useful for those needing to analyze multiple samples simultaneously.
Researchers who require exceptionally high resolution for very small DNA fragments might prefer thinner combs. Individuals without the VWR Midi Plus Horizontal Electrophoresis Systems should consider other options compatible with their existing equipment.
Conclusion on VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb
The VWR Midi Plus Horizontal Electrophoresis Systems E1115-14MC-1 Combs 1.5 Mm x 14-Tooth Comb is a worthwhile investment for labs using the VWR Midi Plus system. Its robust design, ease of use, and ability to create consistent wells make it a valuable tool for improving electrophoresis workflows.
The price of $339.99 is justified by the comb’s durability and the time savings it provides through increased throughput. I would personally recommend it to anyone seeking a reliable and efficient comb for their VWR Midi Plus Horizontal Electrophoresis Systems.
Upgrade your electrophoresis setup today and experience the difference!