Let’s Dive into VWR Maxi 20 Electrophoresis System E1120-20MC0.7 Combs 0.75 Mm x 20-Tooth Comb, Each
The VWR Maxi 20 Electrophoresis System E1120-20MC0.7 Combs 0.75 Mm x 20-Tooth Comb, Each from VWR promises high-throughput screening and high resolution for electrophoresis applications. Its compatibility with 20x20cm gels and durable construction suggests a reliable tool for labs processing a large number of samples. The VWR system aims to streamline PCR product analysis, RFLP analysis, and plasmid screenings in a user-friendly format, but does it deliver on these promises in the real world?
My work in a high-volume research lab meant I was constantly battling throughput issues with our existing electrophoresis setups. The need to efficiently analyze PCR products from 96-well plates became a bottleneck, pushing me to seek a system optimized for speed and resolution. Many systems claimed high throughput, but I wanted one that balanced sample capacity with ease of use and consistent results, which is what brought me to the VWR Maxi 20.
Unboxing the system, I was immediately struck by the robust feel of the acrylic gel box and tray. The components seemed well-made and ready to withstand the daily rigors of lab use. The tray’s UV transparency was a welcome feature, promising easier sample visualization.
Compared to the Hoefer SE600 and Bio-Rad Mini-PROTEAN Tetra system we were using previously, the VWR Maxi 20 offered a larger gel area, theoretically allowing for more samples per run. I chose the VWR Maxi 20 hoping its design would reduce the gel preparation and run time compared to those smaller, older units. The VWR Maxi 20’s ability to accommodate large sample numbers from 96-well plates, seemed more efficient than the other systems.
My first impression was cautiously optimistic. The build quality was encouraging, and the design hinted at improved throughput. I was eager to see if it could live up to the hype in terms of speed and resolution.
Real-World Testing: Putting VWR Maxi 20 Electrophoresis System E1120-20MC0.7 Combs 0.75 Mm x 20-Tooth Comb, Each to the Test
First Use Experience
The initial testing of the VWR Maxi 20 took place in our molecular biology lab, processing PCR products from several 96-well plates. We ran multiple gels with varying DNA concentrations and fragment sizes to assess the system’s resolution and capacity. The 0.75 mm x 20-Tooth Comb seemed well-suited for our standard sample volumes.
The system performed admirably in terms of resolution, separating DNA fragments clearly and consistently. The visibility was good, even with smaller or low-concentration DNA bands. The gel box and tray were easy to set up.
Initially, the learning curve was minimal, thanks to the straightforward design. The casting gates were easy to use. However, I did initially slightly misaligned the comb when setting the gels, resulting in a few wells that needed to be redone.
Extended Use & Reliability
After several weeks of continuous use, the VWR Maxi 20 proved to be a reliable workhorse in our lab. We consistently ran multiple gels per day, analyzing various samples from different experiments. The system held up well under this heavy workload.
The acrylic gel box and tray showed no signs of wear and tear, even after repeated exposure to buffers and cleaning solutions. The platinum electrode cassettes remained easy to clean. The VWR Maxi 20 proved a durable system.
Maintaining the system was relatively simple. Routine cleaning after each run was all that was required to keep it in good working order. The “EasyLift” gel box lid helped to reduce condensation on the gel.
Compared to our previous electrophoresis systems, the VWR Maxi 20 definitely outperformed them in terms of throughput and resolution. The larger gel area allowed us to analyze more samples per run. It resulted in faster turnaround times and reduced the overall workload.
Breaking Down the Features of VWR Maxi 20 Electrophoresis System E1120-20MC0.7 Combs 0.75 Mm x 20-Tooth Comb, Each
Specifications
The VWR Maxi 20 Electrophoresis System E1120-20MC0.7 Combs 0.75 Mm x 20-Tooth Comb, Each features dimensions of 23W x 39.5L x 9H cm (91/16 x 159/16 x 31/2“). This size is ideal for a high-throughput electrophoresis system, offering enough gel surface to analyze multiple samples simultaneously. The large gel area allows for efficient sample processing.
The buffer volume is 1200 mL, which provides ample buffer capacity to maintain stable running conditions. This large volume reduces the need for frequent buffer changes during longer runs. The large buffer volume contributes to consistent results.
The system boasts a max sample capacity of 450, enabled by the use of multiple combs such as the included 0.75 mm x 20-Tooth Comb. This impressive capacity makes it well-suited for labs processing a high volume of samples. The high sample capacity dramatically increases efficiency.
These specifications are essential for the performance and overall user experience. The larger gel dimensions mean more sample can be loaded per run. The larger buffer volume helps maintain consistent conditions and reduces the need for intervention.
Performance & Functionality
The VWR Maxi 20 performs its job exceptionally well, providing accurate and reliable electrophoresis results. The resolution is excellent, allowing for clear separation of DNA fragments of varying sizes. The system is also easy to use, even for inexperienced lab personnel.
The main strength of the VWR Maxi 20 is its high throughput capabilities. The large gel area and multiple comb options make it possible to analyze a large number of samples simultaneously. One potential weakness lies in the initial gel casting process.
The system definitely meets expectations. Its throughput, resolution, and ease of use combine to create a powerful tool for molecular biology labs. The VWR Maxi 20 is reliable and produces excellent results.
Design & Ergonomics
The VWR Maxi 20 features a robust design. The acrylic gel box and tray are well-made and feel durable. The system is also relatively lightweight and easy to move around the lab.
The design is user-friendly, with intuitive controls and a clear layout. There is a slight learning curve associated with the gel casting process. Overall, the VWR Maxi 20 has great ergonomics.
Durability & Maintenance
The VWR Maxi 20 appears to be built to last. The components are made from high-quality materials that can withstand the rigors of daily use. I expect it to last for many years with proper care and maintenance.
Maintaining the system is relatively simple. Routine cleaning after each use is typically sufficient to keep it in good working order. The platinum electrode cassettes are easy to remove. The VWR Maxi 20 is easy to maintain.
Accessories and Customization Options
The VWR Maxi 20 Electrophoresis System comes with one 20x20cm (77/8×77/8″) gel tray, two 20-tooth, 1.5mm thick combs, two 40-tooth, 1.5mm thick combs, and well visualization strips. This set of included accessories allows users to begin running samples immediately.
While the system itself doesn’t offer a wide range of customization options, users can easily swap out the combs for different tooth configurations to suit their specific needs. The gel tray is a standard size, and can be easily found elsewhere. The ability to swap out combs makes the system more versatile.
Pros and Cons of VWR Maxi 20 Electrophoresis System E1120-20MC0.7 Combs 0.75 Mm x 20-Tooth Comb, Each
Pros
- High throughput: Large gel area and multiple comb options allow for efficient analysis of a large number of samples.
- Excellent resolution: Provides clear separation of DNA fragments of varying sizes.
- User-friendly design: Easy to set up and use, even for inexperienced lab personnel.
- Durable construction: The acrylic gel box and tray are well-made and feel durable.
- Easy to maintain: Routine cleaning is typically sufficient to keep it in good working order.
Cons
- Gel casting can be a bit tricky initially.
- Limited customization options beyond comb selection.
Who Should Buy VWR Maxi 20 Electrophoresis System E1120-20MC0.7 Combs 0.75 Mm x 20-Tooth Comb, Each?
The VWR Maxi 20 Electrophoresis System is perfect for molecular biology labs, genetic research facilities, and any research environment needing high-throughput DNA analysis. Labs dealing with PCR products, RFLP analysis, or plasmid screenings will find the system particularly beneficial.
This product may not be ideal for small labs with low sample throughput needs. It might also not be useful for those needing very specialized electrophoresis techniques that require specific equipment.
Must-have accessories include additional combs with different tooth configurations to accommodate varying sample volumes. Purchasing a high-quality power supply to ensure consistent voltage and current delivery is also recommended.
Conclusion on VWR Maxi 20 Electrophoresis System E1120-20MC0.7 Combs 0.75 Mm x 20-Tooth Comb, Each
The VWR Maxi 20 Electrophoresis System E1120-20MC0.7 Combs 0.75 Mm x 20-Tooth Comb, Each is a powerful and reliable tool for high-throughput DNA analysis. Its large gel area, excellent resolution, and user-friendly design make it a valuable asset for any molecular biology lab. While the initial gel casting process can be a bit tricky, the benefits far outweigh this minor inconvenience.
At $209.99, the price is justified by the system’s performance, durability, and high-throughput capabilities. Compared to other electrophoresis systems on the market, the VWR Maxi 20 offers excellent value for the money.
I would definitely recommend the VWR Maxi 20 to any lab looking to improve its DNA analysis workflow. Its high throughput and reliable performance make it a worthwhile investment. Invest in the VWR Maxi 20 and experience the difference in your lab’s productivity!