Let’s Dive into VWR L Blot Box Pk10 E2110-LBB-VW
The VWR L Blot Box Pk10 E2110-LBB-VW, manufactured by VWR, promises a standardized and efficient solution for blot processing in life science research. This pack of ten large blot boxes, each with a 6 ml capacity and designed for 10 x 10 cm blots, caught my attention with its promise of streamlining a critical lab process. The listed price of $249.99 made me consider its potential value in terms of time saved, consistency, and reduced reagent consumption, making the VWR L Blot Box Pk10 E2110-LBB-VW an interesting test subject for an expert review.
My primary need was to find a reliable and consistent method for staining and washing Western blots. I was tired of inconsistent results from manual rocking and shaking in Tupperware containers.
My initial thought upon seeing the VWR L Blot Box Pk10 E2110-LBB-VW was that the design was simple, functional, and likely space-saving. The plastic material appeared durable enough for repeated use with standard laboratory chemicals. Compared to using individual dishes or manually constructed setups, I anticipated these blot boxes would offer a more controlled and streamlined process, potentially saving time and resources.
I considered options like individual rocking platforms or specialized blot processing machines before deciding on the VWR L Blot Box Pk10 E2110-LBB-VW. The other options were more expensive and seemed overkill for the throughput in my current lab setting. My initial impression was cautiously optimistic; I was eager to see if the VWR L Blot Box Pk10 E2110-LBB-VW would live up to its promise of simplifying blot processing.
Real-World Testing: Putting VWR L Blot Box Pk10 E2110-LBB-VW to the Test
First Use Experience
I tested the VWR L Blot Box Pk10 E2110-LBB-VW in a standard molecular biology laboratory setting during antibody incubations for Western blotting. The blot boxes were used on a standard orbital shaker at moderate speed.
The blot boxes performed adequately in various conditions, from room temperature incubations to overnight 4°C antibody binding. The tight-fitting lids prevented evaporation, even during prolonged incubations. The rectangular shape of the VWR L Blot Box Pk10 E2110-LBB-VW did require some getting used to; the corners held onto a bit more liquid than I initially expected when removing reagents.
I encountered no immediate issues, but was initially concerned about the evenness of reagent distribution across the blot area. With the shaker running, it turned out to be a non-issue for the most part.
Extended Use & Reliability
After several weeks of daily use, the VWR L Blot Box Pk10 E2110-LBB-VW boxes have generally held up well. The plastic material has shown no signs of degradation or cracking.
There has been no significant wear and tear, despite frequent exposure to various buffers and staining solutions. The boxes are simple to clean with standard laboratory detergents and deionized water. My previous method involved constantly adjusting volumes of reagents, and the VWR L Blot Box Pk10 E2110-LBB-VW outperforms this by providing a consistent and contained environment.
Breaking Down the Features of VWR L Blot Box Pk10 E2110-LBB-VW
Specifications
- The VWR L Blot Box Pk10 E2110-LBB-VW has a catalog number of 95057-442. Its type is classified as PL LIFE SCIENCE.
- Each box has a 6 ml capacity, suitable for 10 x 10 cm blots. This capacity helps ensure complete blot coverage while minimizing reagent usage.
The specification that it’s a “PL LIFE SCIENCE” type indicates this product is intended for life science laboratory research applications. The dimensions and volume are well-suited for standard Western blotting procedures, providing adequate space and reagent volume for efficient antibody binding and washing steps.
Performance & Functionality
The VWR L Blot Box Pk10 E2110-LBB-VW boxes perform their job adequately. The uniform size and shape contribute to more consistent staining and washing results compared to manual methods.
A strength is their simplicity and ease of use; a minor weakness is the potential for reagent to accumulate in the corners. These blot boxes meet the basic expectations for containment and standardization.
Design & Ergonomics
The boxes are constructed from durable plastic that withstands typical laboratory chemicals. Their lightweight design makes them easy to handle and move around the lab.
The design is straightforward, and there is virtually no learning curve. The VWR L Blot Box Pk10 E2110-LBB-VW offers a simple solution for containment.
Durability & Maintenance
With proper care and cleaning, these boxes should last for a considerable time. The plastic material is resistant to most common laboratory chemicals.
The smooth surfaces make the boxes relatively easy to clean. Abrasive cleaners should be avoided.
Accessories and Customization Options
No accessories are included with the VWR L Blot Box Pk10 E2110-LBB-VW. No customization options are available.
The boxes are designed for use with standard laboratory equipment like orbital shakers. They can be used with blotting paper and membranes from various brands.
Pros and Cons of VWR L Blot Box Pk10 E2110-LBB-VW
Pros
- Provides a consistent and standardized environment for blot processing.
- Helps minimize reagent usage due to the optimized 6 ml capacity.
- Durable plastic construction ensures longevity with proper care.
- The tight-fitting lids prevent evaporation, maintaining consistent reagent concentrations.
- Space-saving design is ideal for labs with limited bench space.
Cons
- Corners can trap small amounts of reagent, potentially leading to uneven staining if not addressed.
- The initial cost of $249.99 for a pack of ten might be a barrier for smaller labs.
Who Should Buy VWR L Blot Box Pk10 E2110-LBB-VW?
Perfect for molecular biology researchers, biochemists, and any lab performing frequent Western blots. Any researcher seeking consistent and standardized blot processing will find these boxes beneficial.
Labs that only perform occasional blots might find the investment unnecessary. Also, those who require highly specialized blotting conditions (e.g., very large blots) may need alternative solutions. Must-have accessories would be an orbital shaker for optimal reagent distribution and standard laboratory detergents for cleaning.
Conclusion on VWR L Blot Box Pk10 E2110-LBB-VW
The VWR L Blot Box Pk10 E2110-LBB-VW offers a practical and reliable solution for standardized blot processing in life science research. Its benefits lie in creating a consistent environment, minimizing reagent waste, and maximizing bench space.
The price of $249.99 may seem steep initially, but the long-term benefits of time saved and improved blot consistency justify the cost for labs performing frequent Western blots. I would recommend the VWR L Blot Box Pk10 E2110-LBB-VW to any lab looking to streamline and standardize their blotting procedures. Consider the VWR L Blot Box Pk10 E2110-LBB-VW to upgrade your lab’s Western blotting efficiency and consistency; it’s an investment in reliable results.