Let’s Dive into VWR Comb 12 Well 1MM Page E2110-12-1-V
The VWR Comb 12 Well 1MM Page E2110-12-1-V is a seemingly simple piece of lab equipment, but its impact on efficient and contamination-free gel electrophoresis is significant. Made by VWR, this comb is designed to create 12 wells, each 1mm wide, in an agarose gel, primarily for DNA or protein sample loading. For researchers who regularly perform gel electrophoresis, the quality and design of the comb can drastically affect the consistency and accuracy of results.
My research lab recently switched to higher throughput screening, demanding a more efficient way to load multiple samples simultaneously. The old combs we were using were inconsistent, often leading to well deformities and cross-contamination. After much searching, I landed on the VWR Comb 12 Well 1MM Page E2110-12-1-V, hoping it would solve these nagging issues.
Unboxing the VWR Comb 12 Well 1MM Page E2110-12-1-V revealed a surprisingly robust and well-finished product. The comb felt solid, with evenly spaced teeth, and no visible imperfections. Its weight provided a sense of quality, indicating a durable construction meant to withstand repeated use and cleaning.
Compared to the generic combs we previously used and a couple of competitor models from Fisher Scientific and Bio-Rad, the VWR Comb 12 Well 1MM Page E2110-12-1-V immediately stood out. The VWR comb appeared to be manufactured with more precision; the teeth were uniformly sized and perfectly aligned, unlike some of the others that had slight variations. Its build quality gave me confidence that it would produce consistent wells and minimize cross-contamination issues.
My first impression was overwhelmingly positive; the VWR Comb 12 Well 1MM Page E2110-12-1-V looked and felt like a serious upgrade. I was excited to see if it could live up to its promise of increased efficiency and reduced contamination, a much-needed boost to our lab’s workflow.
Real-World Testing: Putting VWR Comb 12 Well 1MM Page E2110-12-1-V to the Test
First Use Experience
I immediately integrated the VWR Comb 12 Well 1MM Page E2110-12-1-V into our standard gel electrophoresis protocol. The first test involved running multiple PCR amplicons to verify successful gene amplification.
The comb performed flawlessly under standard lab conditions. Inserting the comb into the cooling agarose and letting it solidify was seamless. Removing the comb after solidification yielded twelve perfectly formed wells, consistent in size and shape.
The comb proved remarkably easy to use. The smooth finish allowed for clean separation from the agarose, and the standardized 1mm width was perfect for our loading volumes. There were no unexpected issues; its user-friendly design ensured minimal disruption to our existing workflow.
Extended Use & Reliability
After several weeks of rigorous daily use, the VWR Comb 12 Well 1MM Page E2110-12-1-V has proven to be a dependable and durable lab tool. There are no signs of wear and tear, even after numerous cycles of agarose solidification and cleaning with ethanol.
Maintaining the VWR Comb 12 Well 1MM Page E2110-12-1-V is incredibly simple. A quick rinse with distilled water followed by an ethanol wipe is all it takes to keep it clean and contamination-free.
In comparison to our previous generic combs, the VWR Comb 12 Well 1MM Page E2110-12-1-V has significantly improved our gel electrophoresis results. The consistent well formation has led to more reliable and reproducible band resolution, exceeding my initial expectations.
Breaking Down the Features of VWR Comb 12 Well 1MM Page E2110-12-1-V
Specifications
The VWR Comb 12 Well 1MM Page E2110-12-1-V is specifically designed for creating twelve wells in an agarose gel, each measuring 1mm in width. This well width is ideal for standard DNA and protein sample loading volumes, minimizing sample diffusion and maximizing resolution. The comb is constructed from a durable, chemical-resistant plastic, likely a polypropylene or similar polymer, capable of withstanding repeated use and cleaning with common lab solvents.
The comb’s precise dimensions and robust material are critical for ensuring consistent well formation and preventing cross-contamination. A comb that bends or deforms easily can lead to uneven well depths and widths, compromising the integrity of the experiment and potentially skewing results. The 1mm well width is optimal for loading typical sample volumes, preventing overflow and promoting sharp band resolution.
Performance & Functionality
The VWR Comb 12 Well 1MM Page E2110-12-1-V performs its core function exceptionally well. It consistently creates uniform wells, contributing to accurate and reliable gel electrophoresis results.
While the comb performs admirably, there is always room for slight improvement. Perhaps adding etched markings to indicate well numbers could enhance usability further.
It unequivocally meets my expectations. The VWR Comb 12 Well 1MM Page E2110-12-1-V has significantly streamlined our workflow and enhanced the reliability of our gel electrophoresis experiments.
Design & Ergonomics
The VWR Comb 12 Well 1MM Page E2110-12-1-V boasts a simple, yet effective design. The comb is constructed of a lightweight yet sturdy plastic, making it easy to handle and maneuver.
The VWR Comb 12 Well 1MM Page E2110-12-1-V is incredibly user-friendly, requiring virtually no learning curve. Its straightforward design makes it accessible even to novice researchers.
Durability & Maintenance
The VWR Comb 12 Well 1MM Page E2110-12-1-V is built to last, showing no signs of degradation even after extensive use and cleaning. Its robust construction ensures it can withstand the rigors of a busy lab environment.
Maintaining the comb is incredibly straightforward. Simply rinse with distilled water and wipe with ethanol to remove any agarose or sample residue.
Accessories and Customization Options
The VWR Comb 12 Well 1MM Page E2110-12-1-V comes as a standalone unit, without any additional accessories. There are no specific customization options offered directly by VWR.
Although there are no direct customizations, its standard design ensures compatibility with most electrophoresis systems. Users could potentially modify the comb’s handles for improved grip, though this is generally unnecessary.
Pros and Cons of VWR Comb 12 Well 1MM Page E2110-12-1-V
Pros
- Consistent Well Formation: Creates uniform, 1mm wells essential for reliable results.
- Durable Construction: Made from chemical-resistant plastic for long-lasting use.
- Easy to Clean: Simple maintenance with distilled water and ethanol.
- Improved Workflow: Streamlines sample loading for efficient gel electrophoresis.
- Enhanced Accuracy: Minimizes cross-contamination and maximizes band resolution.
Cons
- Price Point: Relatively expensive compared to generic combs.
- Lack of Markings: No well number markings for easy sample tracking.
Who Should Buy VWR Comb 12 Well 1MM Page E2110-12-1-V?
The VWR Comb 12 Well 1MM Page E2110-12-1-V is perfect for research labs, biotechnology companies, and educational institutions. It’s ideal for anyone who regularly performs gel electrophoresis and needs reliable, consistent results.
Anyone on a tight budget or who only performs gel electrophoresis occasionally might consider a cheaper, generic alternative. However, the long-term cost savings from reduced experimental errors and improved efficiency likely justify the investment for regular users.
A must-have accessory would be a high-quality electrophoresis system to fully capitalize on the comb’s capabilities. Labeling tape or a fine-tipped marker could be used to add well number markings for easier sample tracking.
Conclusion on VWR Comb 12 Well 1MM Page E2110-12-1-V
Overall, the VWR Comb 12 Well 1MM Page E2110-12-1-V is an excellent investment for any lab seeking to improve the efficiency and reliability of their gel electrophoresis procedures. Its durable construction, consistent well formation, and ease of use make it a valuable addition to any molecular biology toolkit.
While the price may be higher than generic alternatives, the improved results and reduced risk of contamination more than justify the cost. I personally recommend the VWR Comb 12 Well 1MM Page E2110-12-1-V to any researcher looking to upgrade their gel electrophoresis workflow.
If you are serious about your research and want consistent, reliable results, don’t settle for subpar equipment. Invest in the VWR Comb 12 Well 1MM Page E2110-12-1-V and experience the difference a quality comb can make!