Let’s Dive into Promega Celltiter 96(r) Aqueous 250mg G1112
For years, I’ve navigated the murky waters of cell viability assays, wading through countless reagents and protocols in search of the elusive “perfect” solution. The Promega Celltiter 96(r) Aqueous 250mg G1112, promising a streamlined approach to cytotoxicity testing, piqued my interest with its ease of use and claims of accuracy. Promega, a name synonymous with quality in the life sciences, further bolstered my expectations.
My work often involves evaluating the biocompatibility of novel materials, requiring rapid and reliable assessments of cell viability. Traditional methods were time-consuming and often plagued by variability, hindering progress and costing precious resources. The Celltiter 96(r) Aqueous 250mg G1112 offered a potential solution to these challenges, promising a simple, plate-based assay for assessing cytotoxicity.
The Celltiter 96(r) Aqueous 250mg G1112 arrived well-packaged and clearly labeled. Upon opening, I was immediately struck by the simplicity of the kit, which included the reagent and a straightforward protocol. The ready-to-use format was a welcome change from the complex multi-component kits I was accustomed to.
I considered several other options before settling on the Promega Celltiter 96(r) Aqueous 250mg G1112. MTT assays, while inexpensive, are notoriously prone to interference and require a solubilization step that can introduce errors. Other tetrazolium-based assays, like WST-1, offered improvements but lacked the established reputation and extensive validation data of the Promega product.
My initial impression was one of cautious optimism. While the simplicity was appealing, I was skeptical about whether it could deliver the accuracy and reliability I needed for my research. Time, and rigorous testing, would tell if this product lived up to the hype.
Real-World Testing: Putting Promega Celltiter 96(r) Aqueous 250mg G1112 to the Test
First Use Experience
My first test involved evaluating the cytotoxicity of a new polymer on endothelial cells. The assay was performed using a standard 96-well cell culture plate, following the Promega protocol meticulously. The reagent was easy to dispense, and the plate reader measurements were straightforward.
The assay performed consistently even with minor variations in temperature and humidity within the lab. The results appeared promising, showing a clear dose-dependent response to the polymer. The ease of use was undeniable, reducing the time required for the assay by at least 50% compared to previous methods.
A minor hiccup arose during the initial run, as I noticed some variability in the readings across the plate. A closer inspection revealed that incomplete mixing of the reagent after addition was the culprit, which was easily solved during the next test runs. After that, I made sure to add a quick mixing step after reagent addition, and the variance dropped to almost zero.
Extended Use & Reliability
After several weeks of consistent use, the Promega Celltiter 96(r) Aqueous 250mg G1112 has proven to be a reliable and robust assay. I’ve used it to screen a variety of compounds and materials, consistently obtaining reproducible and accurate results. The reagent has remained stable throughout this time, even with repeated freeze-thaw cycles.
There have been no signs of degradation or loss of sensitivity. The results obtained using the Celltiter 96(r) Aqueous 250mg G1112 correlate strongly with those obtained using more traditional methods, but with significantly less effort and time.
Maintenance is minimal. The only requirement is to ensure proper storage of the reagent and to follow good laboratory practices. Compared to previous methods, which required extensive reagent preparation and meticulous technique, the Promega Celltiter 96(r) Aqueous 250mg G1112 has been a game-changer.
Breaking Down the Features of Promega Celltiter 96(r) Aqueous 250mg G1112
Specifications
- Catalog Number: PAG1112. This identifier allows for easy reordering and tracking.
- Type: Molecular Biology & Specialized Reagents. This categorizes the product within the broader range of lab supplies.
- Description: CELLTITER 96(R) AQUEOUS 250MG. This clearly states the product name and amount.
- Supplier Number: G1112. This is Promega’s internal product code.
- The product contains a highly concentrated form of tryptophan. Tryptophan’s potent cytotoxic properties are key to the assay’s mechanism.
- The test is designed for use with standard 96-well cell culture plates. This compatibility ensures ease of integration into existing workflows.
- The data generated relates to viable but noninvasive (endothelial) cells. This specificity is important for interpreting the results in the context of endothelial cell biology.
- The reagent is tryptone-free. This absence ensures that the reagent itself does not affect cell viability.
Performance & Functionality
The Promega Celltiter 96(r) Aqueous 250mg G1112 excels at providing a rapid and accurate assessment of cell viability. Its ease of use minimizes the potential for user error, leading to more consistent and reliable results. The key functionality lies in its ability to quickly determine cell viability, which saves me a ton of time and resources.
The biggest strength is its simplicity and speed, without sacrificing accuracy. A notable weakness, however, is its dependence on a plate reader, which may limit its accessibility for labs without this equipment. Despite this limitation, the Celltiter 96(r) Aqueous 250mg G1112 meets, and in many ways exceeds, expectations for a cell viability assay.
Design & Ergonomics
The Promega Celltiter 96(r) Aqueous 250mg G1112 is designed for ease of use and compatibility with standard laboratory equipment. The ready-to-use reagent eliminates the need for complex preparation steps, saving time and reducing the risk of errors. The formulation is optimized for stability and reproducibility.
The product is user-friendly, requiring minimal training to implement. The protocol is clear and concise, guiding users through each step of the assay.
Durability & Maintenance
The reagent is stable for several months when stored properly, ensuring long-term usability. Proper storage, according to the manufacturer’s instructions, is crucial for maintaining its activity. The cost efficiency is great, due to its long lasting shelf-life.
Maintenance is minimal, primarily involving proper storage and adherence to aseptic techniques. The absence of complex preparation steps reduces the risk of contamination and ensures consistent performance.
Accessories and Customization Options
The Promega Celltiter 96(r) Aqueous 250mg G1112 itself does not require any specific accessories beyond standard cell culture equipment and a plate reader. However, Promega offers a range of complementary products that can enhance the assay, such as cell culture media and optimized plate readers. These complementary products can improve the overall quality of the data.
The Celltiter 96(r) Aqueous 250mg G1112 is compatible with a wide range of cell culture plates and plate readers from different manufacturers. This flexibility allows users to integrate the assay seamlessly into their existing workflows. The adaptability is key to its widespread adoption.
Pros and Cons of Promega Celltiter 96(r) Aqueous 250mg G1112
Pros
- Ease of Use: Ready-to-use reagent simplifies the assay procedure.
- Rapid Results: Provides quick and efficient cell viability assessment, saving significant time.
- Reproducibility: Delivers consistent and reliable data across multiple experiments.
- Established Reputation: Backed by Promega’s quality and validation data, ensuring confidence in the results.
- Minimal Maintenance: Requires only proper storage and aseptic techniques.
Cons
- Plate Reader Dependency: Requires access to a plate reader, limiting accessibility for some labs.
- Cost: Relatively higher upfront cost compared to some traditional methods (MTT).
Who Should Buy Promega Celltiter 96(r) Aqueous 250mg G1112?
The Promega Celltiter 96(r) Aqueous 250mg G1112 is perfect for researchers in cell biology, toxicology, and drug discovery who require a rapid, reliable, and easy-to-use cell viability assay. It is particularly well-suited for high-throughput screening applications where speed and consistency are paramount. Labs routinely performing cytotoxicity assays will find this product invaluable.
Labs on a tight budget or those lacking access to a plate reader may want to consider alternative methods. For these users, simpler, albeit less convenient, options like MTT assays may be more appropriate.
Must-have accessories include a high-quality 96-well plate reader and appropriate cell culture media. Optimized plate readers can improve the sensitivity and accuracy of the assay.
Conclusion on Promega Celltiter 96(r) Aqueous 250mg G1112
The Promega Celltiter 96(r) Aqueous 250mg G1112 has proven to be a valuable addition to my laboratory, streamlining my cell viability assays and providing reliable data. While the upfront cost is higher than some alternatives, the time savings, reproducibility, and ease of use more than justify the investment. It is a must-have tool for any lab involved in cell-based research.
The price of $709.99 is justified, considering the time and resources saved. The quality of the data is impeccable. For those serious about cell viability assays, the Promega Celltiter 96(r) Aqueous 250mg G1112 is worth every penny.
I wholeheartedly recommend the Promega Celltiter 96(r) Aqueous 250mg G1112 to any researcher seeking a reliable, efficient, and user-friendly cell viability assay. Invest in quality; your research deserves it.