Let’s Dive into Pall AcroPak 500, 1000, and 1500 Filter Capsules, Sterile, Pall Life Sciences 12675 Acropak 1500 Filter Capsules
The Pall AcroPak 500, 1000, and 1500 Filter Capsules, Sterile, Pall Life Sciences 12675 Acropak 1500 Filter Capsules represent a crucial piece of equipment for anyone involved in sterile filtration, from lab technicians preparing media to pilot-scale manufacturers seeking reliable and scalable solutions. Manufactured by Pall Life Sciences, these filter capsules are designed for efficient and consistent filtration of volumes ranging from 50L to 150L, ensuring the removal of microorganisms and particulate matter to maintain the integrity of your valuable solutions. The integration of a Supor membrane, known for its high throughput and low protein binding, further enhances their appeal.
My journey to these specific filter capsules stemmed from a persistent challenge: maintaining sterility and achieving consistent flow rates while filtering large volumes of cell culture media. Traditional filtration methods proved time-consuming and often resulted in clogged filters, threatening the viability of my cultures. I wanted a sterile, pre-assembled system that could streamline my workflow and minimize the risk of contamination.
Unboxing the Pall AcroPak 1500 Filter Capsules, I was immediately struck by the robust construction and the clear labeling indicating sterility. The polypropylene housing felt durable, inspiring confidence in its ability to withstand the pressure of filtration. The weight, though substantial, felt appropriate for the filtration capacity advertised.
I had previously used Sartorius Sartopore 2 capsules and Millipore Stericup filters. The Pall AcroPak capsules stood out due to their larger filtration area, potentially leading to faster processing times compared to the Sartorius. I chose the Pall AcroPak for its pre-filtration layer, hoping to extend filter life and improve the throughput of serum-containing media compared to the Stericup’s single-layer membrane.
My first impression was excitement tempered by a touch of apprehension. Could these capsules truly deliver on their promise of high throughput and reliable sterility? The price point was a considerable investment, and I hoped it would translate into significant time savings and improved product quality.
Real-World Testing: Putting Pall AcroPak 500, 1000, and 1500 Filter Capsules, Sterile, Pall Life Sciences 12675 Acropak 1500 Filter Capsules to the Test
First Use Experience
I first tested the Pall AcroPak 1500 Filter Capsules in my cell culture lab, filtering 100L of DMEM media supplemented with 10% fetal bovine serum (FBS). The process involved connecting the capsule to a peristaltic pump and a sterile receiving vessel. I tested the filters indoors at a stable temperature of 22°C.
The capsule performed admirably, maintaining a consistent flow rate even as the FBS-containing media introduced potential clogging agents. The wet conditions from priming the system posed no issue, and the filter maintained its integrity. The ease of use was immediately apparent, requiring minimal setup time.
After initial priming with the media, the filtration process started smoothly, but after about 60L, I noticed a slight decrease in flow rate, indicating some degree of filter loading. No catastrophic failures or unexpected issues arose, however. The pre-filter seemed to be doing its job in prolonging the life of the 0.2µm membrane.
Extended Use & Reliability
After several weeks of consistent use, the Pall AcroPak 1500 Filter Capsules continued to demonstrate their reliability. Over time and multiple filtrations, I noticed no significant degradation in flow rate or structural integrity. The capsules held up well to repeated use.
I observed only minor surface wear on the polypropylene housing after weeks of use; the integrity of the filter membrane itself remained unaffected. The gamma irradiation ensures complete sterility from the get-go.
Cleaning and maintenance were straightforward, involving flushing the capsule with sterile water after each use. Compared to other filtration systems I’ve used, the Pall AcroPak clearly outperforms them in terms of throughput and ease of maintenance. It has become a cornerstone of my lab procedures.
Breaking Down the Features of Pall AcroPak 500, 1000, and 1500 Filter Capsules, Sterile, Pall Life Sciences 12675 Acropak 1500 Filter Capsules
Specifications
- Pore Size: 0.8/0.2 µm – This dual-layer pore size provides both pre-filtration and final sterilization in a single step, enhancing efficiency.
- Pall No.: 12675 – This is the unique identifier for ordering and referencing the AcroPak 1500 capsule specifically.
- Unit: Each – The capsules are sold individually.
- Effective Filtration Area: 500 Series: 500 cm2, 1000 Series: 1000 cm2, 1500 Series: 1500 cm2 – A larger filtration area results in faster flow rates and higher total throughput before clogging occurs, essential for large-volume applications.
- Inlet/Outlet Connections: 500, 1000 Series: 6.4 to 12.7 mm (1/4 to 1/2“) Tapered Hose Barb (Filling Bell on Outlet). 1500 Series: 12.7 mm (1/2“) Straight Hose Barb – The hose barb connections allow for easy integration into existing tubing setups.
- Maximum Operating Temperature: 60°C (140°F) – The maximum temperature allows for filtration of warm solutions while maintaining the integrity of the filter.
- Diameter: 6.9 cm (223/32“) – The diameter contributes to the overall footprint of the filter system.
- Overall Length (without Filling Bell): 500 Series: 14.5 cm (523/32“), 1000 Series: 19.3 cm (73/5“), 1500 Series: 22.9 cm (9”) – The varied lengths provide flexibility for different volume requirements.
Performance & Functionality
The Pall AcroPak 1500 Filter Capsules excel at sterile filtration of large volumes. They provide consistent and reliable performance.
The pre-filtration layer effectively removes particulate matter, extending the life of the final 0.2 µm membrane. Some users may see the cost as a weakness.
The capsule significantly exceeded my expectations in terms of throughput and ease of use. Its sterile design prevents contamination.
Design & Ergonomics
The Pall AcroPak features a robust polypropylene housing, contributing to its durability. The weight is appropriate for its size and filtration capacity.
The capsule is designed for ease of use, with readily accessible hose barb connections. A brief training period is all that’s necessary.
Durability & Maintenance
The robust construction ensures a long lifespan. Routine flushing with sterile water effectively maintains its integrity.
With proper care, the capsules can withstand numerous filtration cycles. This ensures consistent performance over time.
Accessories and Customization Options
The Pall AcroPak 1500 Filter Capsules come pre-sterilized and ready to use. While they don’t offer extensive customization options, the hose barb connections allow seamless integration with various tubing and pumping systems.
The filter can be readily connected to standard laboratory tubing. This ensures compatibility with existing equipment from other brands.
Pros and Cons of Pall AcroPak 500, 1000, and 1500 Filter Capsules, Sterile, Pall Life Sciences 12675 Acropak 1500 Filter Capsules
Pros
- High throughput and flow rates.
- Sterile and ready-to-use, minimizing contamination risks.
- Integrated pre-filter extends the life of the final membrane, saving money long-term.
- Durable polypropylene housing ensures structural integrity.
- Available in multiple sizes to suit varying volume requirements.
Cons
- Relatively high initial cost.
- Limited customization options.
Who Should Buy Pall AcroPak 500, 1000, and 1500 Filter Capsules, Sterile, Pall Life Sciences 12675 Acropak 1500 Filter Capsules?
The Pall AcroPak 500, 1000, and 1500 Filter Capsules, Sterile, Pall Life Sciences 12675 Acropak 1500 Filter Capsules are perfect for laboratory technicians, cell culture specialists, and pilot-scale manufacturers requiring sterile filtration of large volumes. These are ideal for anyone working with cell culture media, protein solutions, or other biopharmaceutical products.
Those working with very small volumes or extremely budget-constrained projects might find cheaper alternatives suitable. If you can’t justify the cost then it may not be for you.
Essential accessories include a peristaltic pump and sterile receiving vessels. Investing in high-quality tubing is also recommended.
Conclusion on Pall AcroPak 500, 1000, and 1500 Filter Capsules, Sterile, Pall Life Sciences 12675 Acropak 1500 Filter Capsules
In summary, the Pall AcroPak 500, 1000, and 1500 Filter Capsules, Sterile, Pall Life Sciences 12675 Acropak 1500 Filter Capsules are a robust and reliable solution for sterile filtration. They are worth the investment.
The price is justified by their high throughput, integrated pre-filter, and ease of use. These factors translate into significant time savings and reduced contamination risks.
I wholeheartedly recommend these filter capsules. They are an invaluable asset for any lab or manufacturing environment where sterility and efficiency are paramount. Invest in the Pall AcroPak 1500, and experience the difference.