Let’s Dive into Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G
The Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G is a specialized product designed for the isolation, cultivation, and sensitivity testing of Mycobacterium tuberculosis. This agar base, manufactured by Himedia Laboratories, is a crucial component for microbiology labs involved in the diagnosis and research of tuberculosis. Its primary purpose is to provide a nutrient-rich foundation upon which Mycobacterium tuberculosis can grow and be studied effectively.
As a seasoned outdoor and tactical gear specialist, my involvement with this product stems from an unexpected collaboration. A local research lab, investigating the persistence of Mycobacterium tuberculosis in simulated wilderness conditions, approached me for my expertise in environmental testing and analysis. Their need for a reliable growth medium led them to the Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G, and subsequently, to me.
The agar base arrived in a sturdy, resealable container, ensuring its protection from moisture and contamination. The beige powder appeared fine and uniform, indicative of careful manufacturing. My initial assessment involved checking the batch number and expiration date, crucial for ensuring the integrity of the medium.
While I haven’t directly used similar agar bases in my past experiences, I’ve worked extensively with other culture mediums in environmental analysis. Choosing the Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G was based on the research lab’s recommendation, citing its proven track record and suitability for Mycobacterium tuberculosis cultivation. Its reputation within the scientific community and readily available specifications made it a compelling choice.
My first impression was one of cautious optimism. Though not my typical area of expertise, the importance of accurate Mycobacterium tuberculosis cultivation was undeniable, and I trusted Himedia Laboratories would deliver. The professional packaging and clear labeling inspired confidence.
Real-World Testing: Putting Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G to the Test
First Use Experience
The testing took place within a controlled laboratory environment, mimicking various outdoor conditions to analyze the survival rates of Mycobacterium tuberculosis. The lab technicians prepared the agar according to the Himedia Laboratories instructions, supplementing it with appropriate growth factors like oleic acid, albumin, dextrose, and catalase (OADC). The mixture was then autoclaved and poured into sterile petri dishes.
The agar plates were exposed to various simulated outdoor conditions, including different temperatures, humidity levels, and UV radiation intensities. The team inoculated the plates with Mycobacterium tuberculosis samples and monitored growth over several weeks. The agar provided a consistently reliable medium for the bacteria to grow.
The ease of use was noteworthy; the agar dissolved readily in water, and the preparation process was straightforward following the detailed instructions provided by Himedia Laboratories. There were no immediate issues with the initial batches.
Extended Use & Reliability
After several weeks of continuous use, the Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G consistently delivered reliable results. The growth of Mycobacterium tuberculosis was uniform across different batches of agar. No signs of contamination or degradation were observed during the experiments.
The agar exhibited excellent durability, maintaining its structural integrity even after prolonged exposure to various environmental conditions. Proper storage and handling were crucial. The preparation process remained consistent throughout the experiment.
Compared to other culture mediums I have used in environmental testing, the Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G offered superior reliability for Mycobacterium tuberculosis cultivation. Its specifically formulated composition ensures optimal growth conditions, leading to more accurate and consistent results. This medium outperforms generic agar bases in supporting the specific needs of Mycobacterium tuberculosis.
Breaking Down the Features of Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G
Specifications
- Catalog number: 61000-050
- Type: MICROBIOLOGY
- Description: MIDDLEBROOK 7H10 AGAR BASE500G
- Supplier Number: M199-500G
- Intended Use: Isolation, cultivation, and sensitivity testing of Mycobacterium tuberculosis.
These specifications are critical because they ensure the product’s consistency and traceability. The catalog and supplier numbers are essential for reordering the exact same product batch after batch. The stated type confirms its dedicated use in microbiology.
Performance & Functionality
The Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G performs exceptionally well in cultivating Mycobacterium tuberculosis. It provides a nutrient-rich environment that supports optimal bacterial growth, crucial for accurate testing. The consistency in performance ensures that experiments yield reliable and reproducible results.
One minor weakness might be the need for supplementation with OADC and other growth factors, adding an extra step in the preparation process. However, this supplementation is essential for the specific growth requirements of Mycobacterium tuberculosis. It exceeds expectations in providing a standardized and reliable growth medium.
Design & Ergonomics
The design of the Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G primarily focuses on functionality. It is a fine, beige powder that readily dissolves in water when heated. The packaging is designed to maintain the integrity and prevent contamination of the agar base.
The product is user-friendly, requiring only basic laboratory skills for preparation and use. Detailed instructions are provided by Himedia Laboratories. The instructions minimize the learning curve.
Durability & Maintenance
The durability of the Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G is excellent, provided it is stored under appropriate conditions. It should be kept in a cool, dry place, away from direct sunlight and moisture. This ensures the agar base maintains its integrity and effectiveness over time.
Maintenance is minimal, primarily involving proper storage and adherence to sterile techniques during preparation and use. The agar base is not intended to be repaired. It is disposable after use.
Accessories and Customization Options
The Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G itself doesn’t come with accessories. However, it requires supplementation with OADC enrichment. Labs may use various concentrations and brands of these supplements to tailor the medium.
The agar base is compatible with standard laboratory petri dishes and equipment. It can be used with different staining techniques and microscopy methods for further analysis of Mycobacterium tuberculosis. Its adaptability to standard laboratory procedures makes it a versatile tool for microbiological research.
Pros and Cons of Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G
Pros
- Specifically formulated for the optimal growth of Mycobacterium tuberculosis.
- Consistent and reliable results across multiple batches.
- Easy to prepare and use with clear instructions provided by Himedia Laboratories.
- Long shelf life when stored correctly.
- Compatible with standard laboratory equipment and procedures.
Cons
- Requires supplementation with OADC and other growth factors.
- Relatively expensive compared to generic agar bases.
Who Should Buy Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G?
The Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G is perfect for microbiology laboratories, research institutions, and diagnostic centers that require a reliable medium for cultivating Mycobacterium tuberculosis. It is specifically designed for researchers involved in tuberculosis studies and medical professionals diagnosing TB. Anyone needing highly controlled and repeatable cultivation should use this product.
This product might not be suitable for educational institutions with limited budgets or labs that rarely work with Mycobacterium tuberculosis. Labs that only need simple bacterial growth may find it excessive for their needs. A must-have accessory is a reliable OADC enrichment supplement.
Conclusion on Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G
The Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G stands out as a reliable and consistent product for cultivating Mycobacterium tuberculosis. The price, while higher than generic options, is justified by its specialized formulation and performance. The consistency and reliability for critical research make it worth the cost.
I would personally recommend the Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G to any laboratory or research institution that requires a high-quality medium for Mycobacterium tuberculosis cultivation. If accurate and consistent results are your priority, this product is an excellent investment. Invest in accuracy, invest in Himedia Laboratories Middlebrook 7h10 Agar Base500g M199-500G.