Let’s Dive into Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300
The Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 is a seemingly simple piece of lab equipment that plays a crucial role in modern molecular biology. These plates are specifically designed for polymerase chain reaction (PCR), a technique that amplifies DNA segments for various research and diagnostic applications. This particular model from Eppendorf features 96 wells, a skirted design for robotic handling, and clear construction, packaged in a pack of 10 sterile plates.
My journey to these plates began with a need for consistent and reliable results in my molecular diagnostics lab. We were experiencing inconsistencies in our PCR amplifications, often traced back to issues with plate warping or contamination. I needed a high-quality, sterile PCR plate solution to minimize these issues and improve the accuracy of our diagnostic assays.
The plates arrived in a well-protected package, each plate individually blistered for optimal sterility. Upon inspection, the design felt robust, and the skirted base provided a stable platform for robotic pipetting systems we use. The clarity of the polypropylene was excellent, allowing for easy visualization of the samples.
Compared to other PCR plates we’ve used from brands like Axygen and Bio-Rad, the Eppendorf plate immediately stood out due to its rigidity and the clarity of the wells. I chose this model specifically for its certified PCR clean and bacterial DNA-free guarantee, addressing my core concern of minimizing contamination. My initial feeling was one of cautious optimism; I was hoping these plates would live up to Eppendorf’s reputation for quality and reliability.
Real-World Testing: Putting Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 to the Test
First Use Experience
I first tested the Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 in our high-throughput diagnostic lab, utilizing an automated liquid handling system for sample preparation. The skirted design of the plate provided excellent stability on the robotic deck, preventing any accidental spills or misalignments. The uniform well dimensions ensured consistent and accurate pipetting across all 96 wells.
We used the plates in a variety of PCR assays, including real-time PCR for viral load quantification and endpoint PCR for genetic mutation screening. The thin-walled construction facilitated rapid and uniform heat transfer, leading to shorter cycling times and more efficient amplification. We observed no issues with plate warping or well distortion, even during prolonged high-temperature incubations.
The ease of use was immediately apparent, requiring no special adjustments to our existing PCR protocols. After the first use, I didn’t encounter any unexpected problems and was pleased with the plates’ performance, and in particular the elimination of previous contamination issues.
Extended Use & Reliability
After several weeks of consistent use, the Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 has proven to be exceptionally reliable. We have not observed any signs of wear or degradation, even with repeated thermal cycling. The plates have consistently maintained their structural integrity, preventing well-to-well contamination.
The clear polypropylene material has resisted discoloration and chemical degradation, even when used with aggressive reagents. Maintenance is minimal; we simply discard the plates after each use, following our standard laboratory protocols for biohazard disposal.
Compared to our previous experiences with cheaper, non-certified PCR plates, the Eppendorf plates have significantly reduced the number of failed PCR runs and false-positive results. The consistent performance and reliability of these plates have justified the higher cost.
Breaking Down the Features of Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300
Specifications
The Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 features:
- 96-well format: This is the standard high-throughput format for PCR, allowing for the simultaneous processing of multiple samples.
- Microbiology grade: This certification ensures the plate is free from bacterial contamination, making it suitable for sensitive PCR applications.
- Skirted design: The skirted base provides added stability and compatibility with robotic handling systems.
- Clear polypropylene: The clear material allows for visual inspection of the wells and optimal light transmission for fluorescence-based assays.
- Sterile: Each plate is individually blistered and sterilized, eliminating the risk of contamination.
- PCR clean certified: This certification guarantees the plate is free from PCR inhibitors and contaminants, ensuring accurate and reliable results.
- Bacterial DNA-free: This feature is crucial for applications requiring the absence of bacterial DNA, such as microbiome studies or diagnostics involving bacterial pathogens.
- Thin-walled wells: The thin walls facilitate rapid and uniform heat transfer, improving PCR efficiency and reducing cycling times.
These specifications are crucial for ensuring reliable and accurate PCR results. The sterility and purity of the plate prevent contamination, while the skirted design and thin-walled wells optimize performance.
Performance & Functionality
The Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 excels in its primary function: providing a reliable and contamination-free platform for PCR. The plates are incredibly accurate, and the well-to-well consistency minimized variability in amplification results.
A significant strength lies in its certified purity, which dramatically reduces the risk of false positives due to contamination. Another strength is the skirted design, which offers stability during robotic handling.
The plates consistently meet and exceed expectations in terms of purity, thermal stability, and robotic compatibility. While the price point is higher than some alternatives, the improved accuracy and reduced error rate justify the investment.
Design & Ergonomics
The Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 is designed with user-friendliness and functionality in mind. The clear polypropylene material provides excellent visibility for sample tracking.
The plate is lightweight yet robust, easily handled manually or by robotic systems. There is no learning curve; the plate integrates seamlessly into existing PCR workflows.
Durability & Maintenance
Given that the Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 is a disposable product, its durability is primarily related to its ability to withstand the rigors of PCR cycling. The high-quality polypropylene construction ensures that the plate will not warp, crack, or leak during thermal cycling.
Maintenance is nonexistent, as the plates are intended for single-use disposal. The individually blistered packaging protects each plate from damage and contamination before use.
Accessories and Customization Options
The Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 is primarily a consumable, and therefore, doesn’t come with accessories in the traditional sense. The plate is designed to be compatible with a wide range of PCR machines and sealing films/foils.
While there aren’t customizable features directly on the plate, its compatibility with standard lab equipment makes it easily adaptable to different PCR workflows. The universal design allows for use with various sealing methods, including adhesive films, heat seals, and cap strips.
Pros and Cons of Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300
Pros
- Certified PCR clean and bacterial DNA-free: This significantly reduces the risk of contamination and false-positive results.
- Skirted design: Provides added stability and compatibility with robotic handling systems.
- Thin-walled wells: Facilitates rapid and uniform heat transfer for efficient PCR.
- Sterile and individually blistered for guaranteed purity.
- Clear polypropylene allows for easy sample visualization.
Cons
- Higher price point compared to non-certified PCR plates.
- Single-use design generates plastic waste, which can be an environmental concern (although standard for PCR).
Who Should Buy Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300?
This Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 is perfect for:
- Researchers performing sensitive PCR assays where contamination is a major concern.
- Diagnostic labs needing reliable and accurate results for clinical testing.
- Laboratories utilizing robotic liquid handling systems for high-throughput PCR.
Who should skip this product?
- Labs on a very tight budget where PCR purity is not a critical requirement.
- Researchers performing basic, non-sensitive PCR assays where contamination is unlikely.
Must-have accessories or modifications?
- Compatible PCR sealing film or foil to prevent evaporation during thermal cycling.
- Appropriate PCR machine capable of accommodating 96-well plates.
Conclusion on Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300
In summary, the Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 is a high-quality, reliable, and contamination-free solution for PCR. Its certified purity, skirted design, and thin-walled wells make it an excellent choice for researchers and diagnostic labs.
The higher price point is justified by the improved accuracy and reduced error rate, ultimately saving time and resources in the long run. The value proposition is clear: reliable results, reduced contamination, and seamless integration into existing workflows.
I would personally highly recommend the Eppendorf Pcr Plate 96wl Mb Skrt Cl Pk10 0030129300 to anyone seeking the best possible performance and reliability in their PCR experiments. Invest in quality, and your results will thank you. Choose Eppendorf for worry-free PCR.