Let’s Dive into Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734
The Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 is a high-throughput PCR plate designed for real-time PCR applications. Manufactured by Eppendorf, a renowned name in laboratory equipment, this plate features a 384-well format, white wells for enhanced signal detection, and a twin.tec design for optimal thermal stability. It comes in a case of 25 plates.
My work demands accuracy and reproducibility when quantifying trace amounts of DNA in environmental samples, where high-throughput screening is crucial. I needed a reliable PCR plate that would minimize well-to-well variability and maximize signal detection, which is especially important when working with low-concentration samples. The Eppendorf reputation for quality and the plate’s white wells for enhanced fluorescence reflection drew me to this product.
Upon unboxing, I immediately noticed the rigidity of the plates, which is reassuring for automated handling. The plates felt sturdy, and the wells were uniformly shaped and spaced, indicating precise manufacturing. The white color of the wells was noticeably brighter than some of the translucent PCR plates I’ve used before, suggesting a potential for better signal reflection.
Compared to other 384-well PCR plates, such as those from Bio-Rad or Thermo Fisher, the Eppendorf plate stood out because of its twin.tec design. This feature promised improved thermal stability and reduced warping during thermal cycling, addressing a common issue I’ve encountered with thinner, less robust plates. The price point was higher than some alternatives, but the potential for increased accuracy and reduced experimental variability seemed worth the investment.
My first impression was one of cautious optimism. While the price seemed steep, the build quality and features hinted at a product that could significantly improve the reliability and efficiency of my high-throughput PCR assays. I was excited to see if the Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 would live up to its promise in the lab.
Real-World Testing: Putting Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 to the Test
First Use Experience
I tested the Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 in our molecular biology lab, specifically for quantifying microbial DNA extracted from soil samples. I used the plate with our standard real-time PCR assay, which involves multiple temperature cycles ranging from 50°C to 95°C.
The plate performed exceptionally well under these conditions. There was no noticeable warping or deformation of the plate during thermal cycling, and the white wells provided a clear and strong fluorescent signal. The automated pipetting system worked smoothly with the plate, thanks to its rigid construction and consistent well spacing.
The plate required no unusual adjustments or familiarization. It fit perfectly into the thermal cycler, and the well design allowed for easy and accurate dispensing of reagents. There were no unexpected issues, such as well leakage or uneven temperature distribution, during the initial run.
Extended Use & Reliability
After several weeks of consistent use, the Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 has proven to be a reliable and robust workhorse. The plates consistently maintain their shape and integrity throughout numerous thermal cycling experiments.
There are no signs of wear and tear, despite the frequent handling and exposure to various reagents. The plate surface is resistant to staining and chemical degradation. Cleaning is straightforward; a simple wipe with ethanol is sufficient to remove any spills or residue.
Compared to previously used PCR plates, the Eppendorf plate demonstrates superior thermal stability and signal detection. The well-to-well consistency in Ct values is noticeably improved, leading to more reliable and reproducible results. This performance justifies the higher price point, making it a worthwhile investment for high-throughput, quantitative PCR applications.
Breaking Down the Features of Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734
Specifications
The Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 is specifically designed for real-time PCR applications requiring high throughput and precise temperature control. Its key specifications include a 384-well format, optimizing reaction volume and sample processing efficiency.
The white well color enhances fluorescent signal detection, providing improved sensitivity, especially for low-copy target sequences. The twin.tec design combines a rigid polycarbonate frame with thin-walled polypropylene wells, offering both mechanical stability and efficient heat transfer. Each case contains 25 plates.
These specifications are critical for accurate and reliable real-time PCR results. The 384-well format allows for processing a large number of samples simultaneously, saving time and resources. The white wells maximize signal-to-noise ratio, ensuring accurate quantification even with minimal target DNA.
Performance & Functionality
The Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 performs exceptionally well in its intended application: high-throughput real-time PCR. Its performance is characterized by high accuracy, reliable well-to-well consistency, and ease of use.
The plate’s strengths lie in its optimal thermal stability, enhanced signal detection, and compatibility with automated systems. A minor weakness is the higher price compared to generic plates. It significantly exceeds expectations in terms of data quality and reproducibility.
Design & Ergonomics
The build quality of the Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 is top-notch, reflecting Eppendorf’s commitment to quality. The rigid polycarbonate frame provides structural integrity, preventing warping during thermal cycling.
The plate is user-friendly and requires no special training to use. It fits standard 384-well thermal cyclers and automated liquid handling systems.
Durability & Maintenance
The Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 is designed for long-term use, even under demanding laboratory conditions. Its robust construction ensures minimal degradation over time.
The plate is easy to maintain. Simple cleaning with standard laboratory disinfectants is sufficient.
Accessories and Customization Options
The Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 doesn’t require specific accessories for basic operation. However, it is compatible with various sealing films and foils designed for 384-well plates, depending on the user’s specific needs.
The plate is also compatible with a wide range of thermal cyclers and liquid handling systems from different brands. This universality makes it a versatile option for diverse laboratory setups.
Pros and Cons of Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734
Pros
- Significantly enhanced fluorescent signal detection due to the white wells, resulting in higher sensitivity and accurate quantification.
- The twin.tec design provides superior thermal stability, minimizing warping and ensuring consistent heat transfer across all wells.
- High compatibility with automated liquid handling systems and thermal cyclers, streamlining high-throughput PCR workflows.
- Exceptional well-to-well consistency in Ct values, leading to highly reproducible and reliable results.
- Manufactured by Eppendorf, a trusted brand known for high-quality laboratory consumables, ensuring consistent performance and reliability.
Cons
- The Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 is more expensive compared to generic 384-well PCR plates.
- While robust, the rigid polycarbonate frame might be slightly less flexible than some thinner plate designs, potentially affecting compatibility with older or less precise thermal cyclers.
Who Should Buy Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734?
The Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 is perfect for researchers, scientists, and technicians involved in high-throughput real-time PCR. This includes those working in molecular biology, genetics, diagnostics, and environmental monitoring.
This product is also ideal for laboratories that require highly accurate and reproducible quantification of nucleic acids, especially when working with low-copy targets. It is suited for labs using automated liquid handling systems and thermal cyclers.
Those with limited budgets or who do not require high precision may consider a cheaper alternative. Also, labs using specialized thermal cyclers with unique plate format requirements might need to verify compatibility before purchasing.
A high-quality sealing film or foil specifically designed for PCR is a must-have accessory. This ensures minimal evaporation and prevents cross-contamination.
Conclusion on Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734
The Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 is a premium PCR plate that delivers exceptional performance and reliability for high-throughput real-time PCR applications. Its enhanced signal detection, superior thermal stability, and robust design make it a valuable asset for any molecular biology lab.
The higher price is justified by the improved data quality, increased throughput, and reduced experimental variability. This directly translates to time and resource savings in the long run.
I would highly recommend the Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 to anyone seeking a reliable and high-performing PCR plate for quantitative, high-throughput studies. Invest in the Eppendorf Pcr Plate 384w Tt Rt Wht Cs25 0030132734 and experience the difference in your real-time PCR assays today!