Let’s Dive into Corning BioCoat Cellware, Poly-Lysine, 356691
The Corning BioCoat Cellware, Poly-Lysine, 356691, manufactured by Corning, represents a crucial tool for researchers focused on cell culture. It is designed to enhance cell attachment and proliferation, critical for various biological assays. The poly-lysine coating promotes a strong cell monolayer and aids in neurite outgrowth. I was drawn to this product, hoping to optimize cell adhesion in my research concerning neuronal network development and stability in vitro.
My initial impression of the Corning BioCoat Cellware, Poly-Lysine, 356691 was largely based on its promise of uniformity and stability. Having worked with uncoated cell cultureware and inconsistent homemade coatings, the appeal of a standardized, ready-to-use solution was significant. The weight and build quality are standard for cell culture plates, with the key difference being the proprietary Corning BioCoat Poly-Lysine treatment, which is invisible to the naked eye but integral to its function.
Compared to standard, uncoated plates or even other commercially available coatings like fibronectin, I chose the Corning BioCoat Cellware, Poly-Lysine, 356691 specifically for its established reputation in promoting neuronal adhesion. I had previously experienced issues with neurons detaching from standard plates, and poly-lysine is well-known for enhancing the attachment of negatively charged cells to positively charged surfaces. I also considered pre-coated Matrigel plates but opted for poly-lysine due to its cost-effectiveness and specific suitability for neuronal cell lines.
My first impression was one of cautious optimism. I was excited about the potential to improve cell adhesion and reduce variability in my experiments. However, the high price point ($2099.00) made me wary and increased my expectations regarding its performance. Would it live up to the hype and justify the investment?
Real-World Testing: Putting Corning BioCoat Cellware, Poly-Lysine, 356691 to the Test
First Use Experience
I tested the Corning BioCoat Cellware, Poly-Lysine, 356691 in my cell culture laboratory, specifically with a primary neuronal cell line derived from mouse hippocampus. The primary goal was to assess the degree of cell adhesion compared to uncoated plates under standard culture conditions: 37°C, 5% CO2, and a humidified atmosphere.
The Corning BioCoat Cellware performed exceptionally well, even under stressful conditions involving multiple media changes and extended incubation periods. The cell adhesion was noticeably superior to that observed on uncoated plates, leading to a more confluent and stable monolayer. The Corning BioCoat Cellware significantly reduced cell detachment during media changes, which was a recurring problem with the uncoated plates.
Using the Corning BioCoat Cellware was straightforward. The plates are pre-coated and ready to use straight from the packaging, eliminating the need for time-consuming and often inconsistent coating protocols. There was a learning curve initially, related to optimizing cell seeding density to account for the enhanced adhesion, but this was easily addressed.
Extended Use & Reliability
After several weeks of continuous use, the Corning BioCoat Cellware maintained its consistent performance. The Corning BioCoat Cellware’s enhanced cell adhesion remained robust over multiple experiments. There were no signs of degradation or reduced efficacy of the poly-lysine coating.
The Corning BioCoat Cellware has proven remarkably durable. Even after repeated handling and incubation cycles, the coating remains intact, showing no signs of peeling or flaking. The Corning BioCoat Cellware is easy to handle during normal cell culture handling.
Maintenance involves only standard cell culture practices: regular media changes and visual inspection under a microscope. No special cleaning or handling procedures are required. Compared to my previous experiences with uncoated plates, the Corning BioCoat Cellware has significantly reduced experimental variability and improved the overall success rate of my cell culture assays.
Breaking Down the Features of Corning BioCoat Cellware, Poly-Lysine, 356691
Specifications
The Corning BioCoat Cellware, Poly-Lysine, 356691 comprises several key technical specifications. It includes pre-coated cell culture plates with a uniform poly-L-lysine coating designed to enhance cell attachment. The coating is applied evenly across the entire surface to ensure consistent performance.
The poly-L-lysine molecules are randomly attached to the surface at the amino acid lysine, irrespective of amino acid sequence. The product is stable for at least three months from the date of shipment when stored at 2 to 8°C (36 to 46°F). This stability and uniformity are essential.
Performance & Functionality
The Corning BioCoat Cellware, Poly-Lysine, 356691 excels in its primary function: promoting robust cell adhesion and proliferation. This leads to more reliable and reproducible experimental results. One strength of the Corning BioCoat Cellware is its consistent lot-to-lot performance, minimizing variability.
One potential area for improvement might be expanding the range of available plate formats and coating densities. The product meets my expectations for promoting cell adhesion. However, its high price point might be a barrier for some researchers.
Design & Ergonomics
The Corning BioCoat Cellware maintains the standard design of cell culture plates. The design of the Corning BioCoat Cellware makes it comfortable to hold in hand. The build quality is high, with durable plastic construction that can withstand normal handling.
The Corning BioCoat Cellware is user-friendly, requiring no special skills or training to use. The Corning BioCoat Cellware is a ready-to-use solution.
Durability & Maintenance
With proper handling and storage, the Corning BioCoat Cellware can last for several weeks or months. The stability of the poly-lysine coating ensures consistent performance throughout its shelf life. Maintenance primarily involves preventing contamination and ensuring proper storage conditions (2 to 8°C).
The Corning BioCoat Cellware is easy to maintain. No specific cleaning or repair procedures are necessary, as it is a disposable product.
Accessories and Customization Options
The Corning BioCoat Cellware, Poly-Lysine, 356691 itself doesn’t come with any additional accessories, as it is a self-contained product. However, it is compatible with standard cell culture equipment. These include microscopes, incubators, and cell counters.
There are limited customization options available for the Corning BioCoat Cellware. The product is available in various plate formats (e.g., 6-well, 12-well, 24-well). While not directly customizable, users can combine the Corning BioCoat Cellware with other surface treatments or growth factors to further optimize cell culture conditions.
Pros and Cons of Corning BioCoat Cellware, Poly-Lysine, 356691
Pros
- Enhanced Cell Adhesion: Promotes robust and consistent cell attachment, leading to more reliable experimental results.
- Uniform Coating: Ensures consistent performance across the entire plate surface.
- Ready-to-Use: Eliminates the need for time-consuming and variable coating protocols.
- Lot-to-Lot Consistency: Reduces experimental variability due to consistent manufacturing standards.
- Suitable for Neuronal Cell Culture: Specifically beneficial for promoting neurite outgrowth and neuronal adhesion.
Cons
- High Price: The cost of $2099.00 may be prohibitive for some researchers, particularly those with limited budgets.
- Limited Customization: There are few options for customizing the coating density or surface properties beyond the standard poly-lysine treatment.
Who Should Buy Corning BioCoat Cellware, Poly-Lysine, 356691?
The Corning BioCoat Cellware, Poly-Lysine, 356691 is perfect for researchers and cell culture specialists. The Corning BioCoat Cellware, Poly-Lysine, 356691 is perfect for those who require consistent and reliable cell adhesion, particularly when working with neuronal cell lines or cells that are difficult to adhere to standard surfaces. This product is well-suited for labs conducting high-throughput screening, drug discovery, or basic research on cell behavior.
Researchers on a tight budget or those who don’t require enhanced cell adhesion for their specific applications might want to skip this product. Cheaper alternatives, like standard uncoated plates or in-house coating methods, may suffice.
For optimal results, researchers should consider using the Corning BioCoat Cellware, Poly-Lysine, 356691 with optimized cell culture media and growth factors that are appropriate for their cell type. Also, researchers should optimize the cell seeding density to maximize cell adhesion and proliferation.
Conclusion on Corning BioCoat Cellware, Poly-Lysine, 356691
The Corning BioCoat Cellware, Poly-Lysine, 356691 is a high-quality product that delivers on its promise of enhanced cell adhesion and consistent performance. It has significantly improved the reliability and reproducibility of my cell culture experiments, particularly with neuronal cell lines. Despite the high price point, the value proposition is justified for researchers who require consistent and robust cell attachment.
I would personally recommend the Corning BioCoat Cellware, Poly-Lysine, 356691 to any researcher looking to optimize their cell culture conditions and reduce experimental variability. However, be mindful of the cost and ensure that the benefits justify the investment for your specific application. If consistent and reliable cell adhesion is critical to your research, then this product is worth the investment.