Let’s Dive into BD Plate Pdl 384W Clr Sp Ro PK5 354662
The BD Plate Pdl 384W Clr Sp Ro PK5 354662, manufactured by BD, might seem unassuming at first glance. But its impact on laboratory processes, particularly in cell-based assays and high-throughput screening, is significant. This plate, designed for 384-well applications, offers a reliable and consistent platform for researchers and scientists.
My initial interest in the BD Plate Pdl 384W Clr Sp Ro PK5 354662 stemmed from a need for a high-quality, consistent plate for optimizing cell culture conditions in a research project focused on drug discovery. I was searching for a plate that minimized well-to-well variability and provided an optimal surface for cell attachment and growth. The poly-D-lysine coating promised enhanced cell adhesion, which was crucial for my experiments.
Upon receiving the package, I was immediately impressed by the packaging; the plates were meticulously protected, ensuring they arrived undamaged and sterile. The clear design allows for easy visual inspection of the wells, and the overall build quality exuded professionalism. The weight felt substantial enough to suggest durable construction, while still being easy to handle.
In comparison to other 384-well plates I’ve used in the past, specifically those from Corning and Greiner Bio-One, the BD Plate Pdl 384W Clr Sp Ro PK5 354662 stood out because of its poly-D-lysine coating. This coating enhances cell adhesion, which is critical for my research. The other plates lacked this specific feature, requiring me to pre-coat them myself, adding extra steps and potential for inconsistency.
My first impression was one of excitement and anticipation. Finally, I had a tool that promised to streamline my cell culture process and improve the reliability of my experimental data. The potential for increased efficiency and accuracy in my research was definitely exciting.
Real-World Testing: Putting BD Plate Pdl 384W Clr Sp Ro PK5 354662 to the Test
First Use Experience
My initial testing of the BD Plate Pdl 384W Clr Sp Ro PK5 354662 took place in a controlled laboratory setting. I used the plates for culturing neuronal cells, which are notoriously difficult to adhere to standard plastic surfaces. The experiment involved assessing cell viability and proliferation under different treatment conditions.
The plate performed admirably under these demanding conditions. Even under prolonged incubation periods at 37°C and high humidity, there was no sign of warping or leakage. The cells adhered uniformly across all wells, demonstrating the effectiveness of the poly-D-lysine coating.
The BD Plate Pdl 384W Clr Sp Ro PK5 354662 was extremely easy to use. No special handling was required beyond standard sterile techniques. The clear design and well spacing facilitated accurate pipetting and microscopic observation.
There were no immediate issues or surprises after the initial use. The plates performed precisely as advertised, and the neuronal cells exhibited robust adhesion and healthy growth. This initial success was encouraging and provided a solid foundation for subsequent experiments.
Extended Use & Reliability
After several weeks of continuous use, the BD Plate Pdl 384W Clr Sp Ro PK5 354662 consistently delivered reliable results. Cell adhesion remained strong, and there was no noticeable degradation in performance over time. The plates proved to be a valuable asset in my research workflow.
The BD Plate Pdl 384W Clr Sp Ro PK5 354662 displayed excellent durability. Despite repeated handling and incubation, there were no signs of physical damage or deterioration. The plates maintained their structural integrity and optical clarity.
Cleaning and maintenance were straightforward. The plates could be easily rinsed with sterile water or PBS (Phosphate-Buffered Saline). Autoclaving is not recommended for these plates, but standard sterilization procedures, such as UV irradiation, worked perfectly.
Compared to my previous experiences with uncoated 384-well plates, the BD Plate Pdl 384W Clr Sp Ro PK5 354662 significantly outperformed them in terms of cell adhesion and uniformity. The poly-D-lysine coating eliminated the need for tedious pre-coating steps and enhanced the reproducibility of my experiments.
Breaking Down the Features of BD Plate Pdl 384W Clr Sp Ro PK5 354662
Specifications
The BD Plate Pdl 384W Clr Sp Ro PK5 354662 boasts several key specifications that contribute to its performance:
Well Format: 384-well, designed for high-throughput screening and cell-based assays. This facilitates efficient and simultaneous processing of a large number of samples.
Material: Polystyrene, providing a rigid and optically clear platform for cell culture and analysis. Polystyrene is also compatible with a wide range of solvents and reagents commonly used in laboratory settings.
Coating: Poly-D-Lysine, promoting enhanced cell adhesion and growth. This synthetic molecule mimics the adhesive properties of extracellular matrix components, creating a favorable environment for cell attachment and spreading.
Sterility: Tested for bacteria and fungi, ensuring a sterile environment for cell culture. Sterility is critical for preventing contamination and maintaining the integrity of experimental results.
Packaging: Pack of 5 plates, allowing for cost-effective use and storage. This minimizes waste and ensures that fresh, sterile plates are always available.
Color: Clear, enabling easy visual inspection and microscopic observation of cells. Clear plates also facilitate accurate absorbance and fluorescence measurements in plate readers.
These specifications are essential for ensuring reliable and reproducible results in cell-based assays. The 384-well format enables high-throughput screening, while the poly-D-lysine coating promotes cell adhesion, and the sterility testing prevents contamination.
Performance & Functionality
The BD Plate Pdl 384W Clr Sp Ro PK5 354662 excels in its primary function: providing a reliable platform for cell culture. Cell adhesion is consistently strong, and the plates exhibit minimal well-to-well variability. This translates into more accurate and reproducible experimental data.
The main strength of the BD Plate Pdl 384W Clr Sp Ro PK5 354662 lies in its poly-D-lysine coating. This coating significantly enhances cell adhesion, particularly for cells that are difficult to attach to standard plastic surfaces. One potential area for improvement would be to offer different surface coatings to cater to a wider range of cell types and experimental needs.
The BD Plate Pdl 384W Clr Sp Ro PK5 354662 meets and often exceeds expectations. It delivers consistent performance, simplifies cell culture workflows, and enhances the reliability of experimental data. This makes it a valuable tool for any research laboratory or biotechnology company.
Design & Ergonomics
The BD Plate Pdl 384W Clr Sp Ro PK5 354662 features a user-friendly design. The clear polystyrene material provides excellent optical clarity, and the well spacing facilitates accurate pipetting and microscopic observation. The weight and handling characteristics are also optimal for laboratory use.
The plate requires no special handling or preparation beyond standard sterile techniques. There is virtually no learning curve associated with its use. This makes it easy to integrate into existing laboratory workflows.
Durability & Maintenance
The BD Plate Pdl 384W Clr Sp Ro PK5 354662 is made from durable polystyrene, ensuring a long lifespan under normal laboratory conditions. With proper handling and storage, these plates can be used repeatedly without any noticeable degradation in performance. The plate should be expected to last the life of the experiment.
Maintenance is straightforward. The plates can be easily rinsed with sterile water or PBS. Autoclaving is not recommended, but standard sterilization procedures, such as UV irradiation, are effective for maintaining sterility.
Accessories and Customization Options
The BD Plate Pdl 384W Clr Sp Ro PK5 354662 does not come with any specific accessories. However, it is compatible with a wide range of standard laboratory equipment, such as multi-channel pipettes, plate readers, and automated liquid handling systems. The universal format provides flexibility for any lab setting.
Customization options are limited for this specific product. However, BD offers a variety of other 384-well plates with different surface coatings and treatments to suit diverse experimental needs. Researchers can also explore third-party providers for specialized plate sealers and other accessories.
Pros and Cons of BD Plate Pdl 384W Clr Sp Ro PK5 354662
Pros
- Enhanced Cell Adhesion: The poly-D-lysine coating significantly improves cell attachment and growth, particularly for difficult-to-adhere cell types.
- High-Throughput Compatibility: The 384-well format facilitates efficient screening and high-volume experiments.
- Reliable Performance: The plates exhibit consistent performance and minimal well-to-well variability, leading to more reproducible results.
- Sterile Packaging: The plates are individually tested for sterility, ensuring a contamination-free environment for cell culture.
- Optical Clarity: The clear polystyrene material provides excellent optical clarity for visual inspection and plate reader measurements.
Cons
- Limited Customization: The fixed poly-D-lysine coating may not be suitable for all cell types or experimental needs.
- Price: At $229.99 for a pack of 5, the plates are relatively expensive compared to uncoated plates.
Who Should Buy BD Plate Pdl 384W Clr Sp Ro PK5 354662?
The BD Plate Pdl 384W Clr Sp Ro PK5 354662 is perfect for cell biologists, biochemists, and researchers involved in drug discovery, high-throughput screening, and cell-based assays. Those working with neuronal cells or other cell types that require enhanced adhesion will especially benefit from its poly-D-lysine coating. It would also be perfect for pharmaceutical companies.
Those who should skip this product are researchers who primarily work with suspension cells or cell types that adhere readily to standard plastic surfaces. The poly-D-lysine coating may not be necessary or provide any additional benefit in these cases. Additionally, researchers with very tight budgets may find the price prohibitive.
A must-have accessory would be a reliable plate sealer to prevent evaporation and contamination during long-term incubations. Sterile pipettes and multi-channel pipettes are also essential for efficient handling of the 384-well format.
Conclusion on BD Plate Pdl 384W Clr Sp Ro PK5 354662
The BD Plate Pdl 384W Clr Sp Ro PK5 354662 is a high-quality, reliable tool that delivers exceptional performance in cell-based assays. Its poly-D-lysine coating, high-throughput compatibility, and sterile packaging make it a valuable asset for any research laboratory. While the price may be a barrier for some, the enhanced cell adhesion and improved reproducibility justify the investment for many applications.
The price of $229.99 is justified by the superior performance and enhanced cell adhesion offered by the BD Plate Pdl 384W Clr Sp Ro PK5 354662. This superior adhesion makes it a worthy investment.
I would definitely recommend the BD Plate Pdl 384W Clr Sp Ro PK5 354662 to anyone looking for a high-quality, reliable 384-well plate for cell culture. If you are looking for a great plate for cell culture and high-throughput processing, then look no further. Take your cell culture research to the next level with the BD Plate Pdl 384W Clr Sp Ro PK5 354662!