Let’s Dive into BD Plate Coll I 384W WHT/CLR PK5 354664
The BD Plate Coll I 384W WHT/CLR PK5 354664 from BD is a specialized labware accessory designed for cell culture. Specifically, it’s a 384-well plate coated with Collagen I, promoting enhanced cell attachment and proliferation. Its white/clear design and pack of five make it a valuable tool for high-throughput screening and cell-based assays.
My background is in tactical and outdoor gear, not necessarily lab equipment. But I encountered the need for this specific type of plate while collaborating with a research group developing bio-sensors for field diagnostics. They required consistent and reliable cell cultures, pushing me to explore beyond my usual expertise.
Upon unboxing, the immediate impression is one of precision and quality. The plates are individually sealed within the pack, maintaining sterility. The white wells against the clear body give a crisp, clean look suggesting meticulous manufacturing standards.
Compared to standard polystyrene plates, these BD Plate Coll I 384W WHT/CLR PK5 354664 plates stand out due to the Collagen I coating. This coating is absent in basic cell culture plates, requiring researchers to add it separately. Furthermore, other collagen-coated plates often have inconsistent application, which is problematic.
Initially, I was cautiously optimistic. While the specifications were promising, the real test would be how the plates performed under rigorous cell culture conditions. I hoped they’d deliver on their promise of enhanced cell attachment and growth.
Real-World Testing: Putting BD Plate Coll I 384W WHT/CLR PK5 354664 to the Test
First Use Experience
Our first use involved culturing HT-1080 cells. We compared cell adhesion and proliferation rates on these BD Plate Coll I 384W WHT/CLR PK5 354664 plates against uncoated plates. The BD plates were used in a controlled laboratory setting.
The conditions were standard: 37°C, 5% CO2, and high humidity. Using these plates was straightforward. No special preparation was required besides standard sterile techniques.
The most notable observation was the significantly improved cell attachment compared to the control group. The cells appeared healthier and more evenly distributed across the wells. There were no immediate issues.
Extended Use & Reliability
After several weeks of continuous culturing, the BD Plate Coll I 384W WHT/CLR PK5 354664 plates continued to perform consistently. Cell growth remained robust, and we observed no signs of contamination. The plates held up exceptionally well.
There were no signs of physical degradation or changes in the Collagen I coating. The plates were stored as per BD‘s recommendations and were easy to handle during media changes. In comparison to previous attempts with self-coated plates, the consistent cell growth and uniformity was a vast improvement.
Breaking Down the Features of BD Plate Coll I 384W WHT/CLR PK5 354664
Specifications
The BD Plate Coll I 384W WHT/CLR PK5 354664 specifications are designed for optimal cell culture performance. The plate is a 384-well format, which facilitates high-throughput screening. The plates are white with clear bottoms, ideal for luminescent and fluorescent assays.
The Collagen I coating is derived from rat tail tendon, known for its high purity and biocompatibility. The white wells minimize light scatter, improving the signal-to-noise ratio in optical assays. These features are critical for reliable and reproducible experimental results.
Performance & Functionality
The BD Plate Coll I 384W WHT/CLR PK5 354664 excels in promoting cell adhesion and proliferation. It offers consistent and uniform cell growth across all 384 wells. It meets and exceeds expectations.
The uniformity of the Collagen I coating ensures consistent cell behavior. Any inconsistency can lead to skewed results. This plate’s reliability significantly reduces experimental variability.
Design & Ergonomics
The plates exhibit high-quality construction with sturdy materials. The 384-well format maximizes throughput while maintaining ease of handling. The clear bottom allows for convenient microscopic observation.
The plates are designed for compatibility with automated liquid handling systems. The ergonomics promote efficient and accurate cell culture workflows. There is virtually no learning curve for experienced cell culture technicians.
Durability & Maintenance
The BD Plate Coll I 384W WHT/CLR PK5 354664 is designed for single-use applications to maintain sterility. Proper storage is crucial to ensure the stability of the Collagen I coating. It will likely last a substantial amount of time given the proper storage.
Maintaining sterility during handling is important, but minimal specific maintenance is required. They are disposable, eliminating the need for cleaning or sterilization. The plates should remain stable for at least six months when stored at 4 to 30°C.
Accessories and Customization Options
The BD Plate Coll I 384W WHT/CLR PK5 354664 is inherently a specialized consumable. Therefore, it doesn’t come with traditional accessories. However, its 384-well format makes it compatible with standard robotic handling systems and multi-channel pipettes.
The plate works seamlessly with various cell culture media and supplements. Furthermore, researchers can readily integrate it into existing laboratory workflows. The lack of need for specific accessories showcases its well-designed functionality and purpose.
Pros and Cons of BD Plate Coll I 384W WHT/CLR PK5 354664Pros
- Consistent Collagen I coating promotes reliable cell adhesion and proliferation.
- White wells enhance signal-to-noise ratio in optical assays, improving data quality.
- 384-well format enables high-throughput screening, saving time and resources.
- Sterile packaging ensures contamination-free cell cultures.
- Compatibility with automation streamlines cell culture workflows.
Cons
- The plates are designed for single-use, adding to consumable costs.
- While broadly applicable, the Collagen I coating is optimized for specific cell types.
Who Should Buy BD Plate Coll I 384W WHT/CLR PK5 354664?
Pros
- Consistent Collagen I coating promotes reliable cell adhesion and proliferation.
- White wells enhance signal-to-noise ratio in optical assays, improving data quality.
- 384-well format enables high-throughput screening, saving time and resources.
- Sterile packaging ensures contamination-free cell cultures.
- Compatibility with automation streamlines cell culture workflows.
Cons
- The plates are designed for single-use, adding to consumable costs.
- While broadly applicable, the Collagen I coating is optimized for specific cell types.
The BD Plate Coll I 384W WHT/CLR PK5 354664 is perfect for cell biologists, researchers in drug discovery, and anyone involved in high-throughput cell-based assays. It is ideal for labs prioritizing consistent and reliable cell cultures. It is useful for those requiring accurate optical measurements.
Researchers working with cell types not effectively supported by Collagen I might want to consider alternative plate coatings. Smaller-scale cell culture experiments may not justify the cost and throughput of a 384-well plate. A must-have accessory is sterile cell culture media appropriate for the cells being cultured.
Conclusion on BD Plate Coll I 384W WHT/CLR PK5 354664
The BD Plate Coll I 384W WHT/CLR PK5 354664 delivers exceptional performance and reliability in cell culture applications. The consistent Collagen I coating and optimized design translate to reproducible and high-quality results. Its sterile packaging ensures that each plate is ready for immediate use, minimizing the risk of contamination.
Although the single-use design adds to consumable costs, the benefits in terms of data quality and throughput justify the price. The price is justified due to the quality. I would personally recommend this product to anyone seeking a reliable and high-performance cell culture solution for high-throughput applications.
If you need consistent and high-quality cell cultures, invest in the BD Plate Coll I 384W WHT/CLR PK5 354664 today!