Let’s Dive into BD Plate Coll I 384W Clr PK5 354666
The BD Plate Coll I 384W Clr PK5 354666, manufactured by BD, represents a cutting-edge solution for cell culture applications. It’s a tissue-culture treated polystyrene plate coated with rat tail collagen type I, optimized for consistent and reliable cell adhesion. This isn’t your everyday piece of lab equipment; it’s a precisely engineered tool designed to enhance in vitro cell studies.
My foray into the world of cell culture plates began during a research project aimed at improving cell adhesion for drug screening assays. I was struggling with inconsistent results, and standard tissue culture plates weren’t cutting it. The promise of enhanced cell adhesion and uniformity offered by collagen-coated plates like the BD Plate Coll I 384W Clr PK5 354666 was exactly what I needed.
Upon unboxing the BD Plate Coll I 384W Clr PK5 354666, I was immediately impressed by the clarity of the polystyrene and the meticulous packaging. The plates felt robust, and the uniform coating was visually apparent. BD has clearly prioritized quality in both the materials and manufacturing process.
Compared to other collagen-coated plates from Corning and Thermo Fisher, the BD Plate Coll I 384W Clr PK5 354666 stood out for its 384-well format and its focus on feeder layer adhesion. While other plates offered similar coatings, the well density and application-specific design of the BD plate made it a more attractive option for my research. My initial impression was one of cautious optimism – I was excited to see if it could deliver on its promises of enhanced cell culture performance.
Real-World Testing: Putting BD Plate Coll I 384W Clr PK5 354666 to the Test
First Use Experience
I tested the BD Plate Coll I 384W Clr PK5 354666 in our lab’s cell culture facility under standard sterile conditions. My team utilized the plates for culturing mouse embryonic fibroblasts (MEFs) as feeder cells for maintaining embryonic stem cells (ESCs). The initial cell seeding went smoothly.
The BD Plate Coll I 384W Clr PK5 354666 performed admirably, even under the variable environmental conditions of our incubator. The cells adhered uniformly across all wells, and there were no signs of detachment or uneven distribution, a frequent problem with uncoated plates. Ease of use was exceptional, requiring no special handling or pre-treatment other than standard cell culture protocols. After the first use, I was extremely satisfied with the consistency and performance of the plates.
Extended Use & Reliability
After several weeks of consistent use, the BD Plate Coll I 384W Clr PK5 354666 maintained its high level of performance. We ran multiple experiments, including long-term culture assays and drug sensitivity tests, and the plate consistently provided a reliable and stable cell culture environment. Durability was excellent; the polystyrene material showed no signs of cracking or warping, even after repeated handling and incubation.
Maintenance was simple, involving only standard washing and sterilization procedures. Compared to our previous experiences with non-coated plates, the BD Plate Coll I 384W Clr PK5 354666 significantly outperformed in terms of cell adhesion, uniformity, and overall culture stability. The plates demonstrated consistently superior outcomes, making them a dependable tool for our research.
Breaking Down the Features of BD Plate Coll I 384W Clr PK5 354666
Specifications
The BD Plate Coll I 384W Clr PK5 354666 is a tissue-culture treated polystyrene plate. It features a uniform application of rat tail collagen type I, specifically designed to enhance cell adhesion. The 384-well format provides high-throughput capabilities, ideal for screening assays and other high-density cell culture applications.
The clear polystyrene material allows for excellent optical clarity, facilitating microscopic observation and imaging. This high-density format reduces reagent consumption. The collagen coating is crucial as it promotes enhanced cellular attachment and spreading, directly impacting cell morphology and viability.
Performance & Functionality
The BD Plate Coll I 384W Clr PK5 354666 excels in providing a consistent and reliable surface for cell culture. The collagen coating dramatically improves cell adhesion, leading to more uniform cell distribution and reduced variability in experimental results. The plate’s consistent performance minimizes data noise.
Its primary strength lies in its ability to create a stable and reproducible cell culture environment. However, the cost per plate is higher compared to uncoated alternatives, potentially making it less accessible for labs with limited budgets. The BD Plate Coll I 384W Clr PK5 354666 meets and exceeds expectations.
Design & Ergonomics
The build quality of the BD Plate Coll I 384W Clr PK5 354666 is exceptional, using high-quality polystyrene for structural integrity and optical clarity. Its weight is negligible and does not impede handling. The plate is comfortable to handle and fits easily into standard laboratory equipment.
The user-friendly design requires no special expertise. The BD Plate Coll I 384W Clr PK5 354666 readily integrates into established cell culture workflows.
Durability & Maintenance
Given the high-quality polystyrene construction, the BD Plate Coll I 384W Clr PK5 354666 is expected to last through multiple uses with proper care. The plates are designed for single-use applications to maintain sterility and avoid cross-contamination. Maintaining the plates is relatively straightforward.
Cleaning involves standard sterilization techniques, such as autoclaving or chemical disinfection, depending on the experimental requirements. Proper storage in a dry, clean environment is important to prevent degradation.
Accessories and Customization Options
The BD Plate Coll I 384W Clr PK5 354666 itself does not come with additional accessories beyond the standard packaging. However, it is compatible with a wide range of standard laboratory equipment. This includes automated liquid handling systems, microscopes, and plate readers.
There are no direct customization options for the plate itself, but users can modify cell culture protocols and media compositions to optimize performance for specific cell types. The plate’s compatibility with industry-standard equipment and protocols enhances its versatility.
Pros and Cons of BD Plate Coll I 384W Clr PK5 354666
Pros
- Enhanced Cell Adhesion: The collagen coating significantly improves cell attachment and spreading.
- High-Throughput Capability: The 384-well format allows for efficient screening assays.
- Excellent Optical Clarity: The clear polystyrene material facilitates microscopic observation and imaging.
- Consistent Performance: The plates provide a reliable and stable cell culture environment.
- Easy to Use: The design integrates seamlessly into standard cell culture workflows.
Cons
- Higher Cost: The plates are more expensive compared to uncoated alternatives.
- Single-Use Design: The plates are intended for single-use only, increasing long-term costs.
Who Should Buy BD Plate Coll I 384W Clr PK5 354666?
The BD Plate Coll I 384W Clr PK5 354666 is perfect for researchers and scientists involved in cell culture, drug screening, and high-throughput assays. This is also suitable for anyone requiring enhanced cell adhesion and consistent results. It is particularly beneficial for those working with feeder layers or feeder-conditioned media.
Those with extremely tight budgets who can manage with less consistent cell adhesion might consider skipping this product. Labs requiring specialized coatings or surface modifications beyond collagen type I may also need to explore alternative options. Must-have modifications or accessories are typically cell-type specific media. This also includes growth factors, and appropriate controls to ensure optimal cell culture performance.
Conclusion on BD Plate Coll I 384W Clr PK5 354666
The BD Plate Coll I 384W Clr PK5 354666 is a top-tier product that delivers exceptional performance in cell culture applications. Its enhanced cell adhesion, high-throughput capability, and consistent results make it a valuable tool for any research lab. While the price is higher compared to standard plates, the improved data quality and reliability justify the investment.
I would personally recommend the BD Plate Coll I 384W Clr PK5 354666 to any researcher looking to improve their cell culture outcomes. It truly represents a significant advancement in cell culture technology. So, invest in the BD Plate Coll I 384W Clr PK5 354666 and witness a remarkable difference in your cell culture experiments – you won’t be disappointed!