Let’s Dive into Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S
The Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S, manufactured by Axygen, represents a crucial component in modern laboratory practices, especially where precision and contamination control are paramount. These pre-sterilized, clear filter pipet tips, designed for 300uL volumes and packaged in convenient racks of 96, are engineered with a hydrophobic filter to prevent cross-contamination, making them ideal for sensitive applications like DNA multiplication and handling hazardous materials. Priced at $949.99, the cost reflects the importance of accuracy and safety in critical research and development.
My work increasingly involves PCR amplification and the manipulation of sensitive RNA samples. The threat of aerosol contamination and carry-over is a constant concern that could easily invalidate months of work. I needed a reliable, sterile solution that would provide an effective barrier without compromising pipetting accuracy.
Opening the case, I was immediately struck by the clean, organized arrangement of the racks. Each tip was pristine and perfectly aligned, promising consistency and sterility. The build quality seemed exceptional, exceeding the quality of the generic tips I had used in the past.
Compared to standard, non-filtered pipette tips, the Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S offered an unparalleled level of protection. I also considered Eppendorf filter tips and Rainin filter tips, both reputable brands. Ultimately, I selected the Axygen tips based on the documented filter material and pore size specifications, which seemed best suited for my specific application and offered the best value.
My initial reaction was overwhelmingly positive. The promise of a truly sterile and contamination-free pipetting experience was incredibly exciting, potentially saving countless hours of troubleshooting and re-testing. I felt a surge of confidence that this investment would pay off in the long run.
Real-World Testing: Putting Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S to the Test
First Use Experience
My first real-world test involved setting up a sensitive quantitative PCR (qPCR) assay in our molecular biology lab. The assay aimed to quantify viral load in clinical samples, requiring utmost precision and zero cross-contamination. The Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S tips were used to transfer DNA template, enzymes, and master mix components into the reaction wells.
The lab environment maintained controlled temperature and humidity, but preventing aerosol contamination during pipetting was still critical. Pipetting with the Axygen tips felt natural and intuitive; there wasn’t a noticeable change in aspiration or dispensing behavior compared to standard tips. The hydrophobic filter ensured that no droplets or aerosols entered the pipettor, contributing to my peace of mind.
There were no immediate issues or surprises. Dispensing was smooth and consistent, and the tips fit perfectly on my Eppendorf pipettes. The qPCR results showed excellent reproducibility and no signs of contamination in the negative controls, a clear indication of the tips’ effectiveness.
Extended Use & Reliability
After several months of consistent use, the Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S tips have proven to be incredibly reliable. We’ve used them in various applications, including cDNA synthesis, library preparation for next-generation sequencing, and CRISPR-Cas9 gene editing. The consistent performance across these experiments affirms their quality.
I’ve observed no signs of degradation or filter failure. Each tip maintains its structural integrity, even after prolonged exposure to various reagents. Proper storage in the original racks and controlled humidity have likely contributed to their longevity.
Maintaining these tips is practically effortless, as they are single-use and pre-sterilized. The pre-sterilization eliminates the tedious task of autoclaving, saving valuable time and resources. They have outperformed standard tips in every scenario, significantly reducing the incidence of false positives and ambiguous results.
Breaking Down the Features of Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S
Specifications
- Description: Filter Pipet Tip. This highlights the core function of the product: a specialized pipette tip featuring an integrated filter.
- Filter Type: Hydrophobic filter barrier. This feature is vital for preventing liquid and aerosol contamination, essential for sensitive experiments.
- Application: Ideal for DNA multiplication and microbiological applications, including the handling of radioactive, biohazardous, and corrosive samples. This clearly outlines the specific use cases where the tips excel.
- Contamination Guard: Guard against cross-contamination. This reinforces the primary benefit: preventing inaccurate results due to contamination.
- Tip Type: Universal tip type. This ensures compatibility with a wide range of pipettes, reducing the need for multiple tip types.
- Volume: 300uL. Defines the specific volume capacity of the tip, allowing for accurate measurements.
- Sterility: Pre-sterilized. Eliminates the need for autoclaving and ensures immediate usability for sterile applications.
- Clarity: Clear. Allows for easy visual inspection of the liquid level within the tip.
- Packaging: 96 tips/rack. Convenient packaging for efficient use and storage.
These specifications are important because they directly influence the reliability and accuracy of experimental results. The hydrophobic filter prevents aerosols and liquids from entering the pipette, which could cause cross-contamination. Pre-sterilization eliminates the risk of introducing external contaminants, ensuring accurate results.
Performance & Functionality
The Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S performs its job exceptionally well. Its primary function is to deliver accurate and contamination-free liquid transfers, and it consistently delivers. The universal fit makes it compatible with a variety of pipettes in our lab.
The greatest strength lies in the filter’s effectiveness, virtually eliminating cross-contamination issues. A minor weakness might be the slightly higher cost per tip compared to non-filtered alternatives; however, the peace of mind and reliability are worth the investment. The product significantly exceeds expectations regarding contamination control.
Design & Ergonomics
The tips are constructed from high-quality, clear polypropylene, providing excellent visibility. The material is rigid enough to maintain its shape during pipetting. The lightweight design ensures comfortable handling even during long experiments, reducing user fatigue.
The tips exhibit a user-friendly design with no learning curve. They easily snap onto pipettes and eject effortlessly after use, minimizing hand strain. Overall, the design is simple and effective.
Durability & Maintenance
Given that the Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S tips are single-use disposables, durability primarily refers to the integrity of the filter during use. The filters consistently maintain their integrity, even when handling viscous or volatile liquids. They require no maintenance since they are designed for single-use disposal.
Accessories and Customization Options
The product itself doesn’t offer any customization options in the traditional sense. However, it’s worth noting that the universal fit allows for compatibility with a wide range of pipette brands and models. This makes it compatible with a variety of pipette brands, including Eppendorf, Thermo Fisher, and Rainin. The racks are also autoclavable, allowing for reuse if sterility isn’t strictly required, although this isn’t their intended use.
Pros and Cons of Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S
Pros
- Exceptional contamination control: The hydrophobic filter effectively prevents aerosol and liquid contamination.
- Pre-sterilized convenience: Eliminates the time and effort required for autoclaving.
- Universal fit: Compatible with a wide range of pipette brands and models.
- Consistent performance: Delivers reliable and accurate liquid transfers.
- High-quality materials: Ensures structural integrity and prevents reagent interactions.
Cons
- Higher cost per tip compared to non-filtered options.
- Single-use design may contribute to plastic waste (although recyclable).
Who Should Buy Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S?
The Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S is perfect for molecular biologists, microbiologists, and researchers working with DNA, RNA, infectious agents, or other sensitive materials. It is essential for anyone performing PCR, qPCR, sequencing, or cell culture. Researchers who prioritize accuracy and contamination control will find this product invaluable.
Those on a tight budget and who perform routine, non-sensitive liquid transfers may find standard, non-filtered tips sufficient. Also, labs that have stringent waste disposal requirements may need to carefully consider the single-use nature of these tips. A must-have accessory would be a high-quality pipette to ensure optimal accuracy and precision.
Conclusion on Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S
The Axygen Tip Filter 350UL Clr St PK960 TF-350-R-S is an outstanding product that delivers on its promise of providing accurate, reliable, and contamination-free liquid transfers. While the price is higher than standard tips, the investment is justified for applications where accuracy and reproducibility are paramount. I wholeheartedly recommend these tips to any researcher who values the integrity of their experiments. If you’re tired of questionable results and the constant worry of contamination, invest in these tips, and you’ll experience the peace of mind that comes with using a truly reliable product.