Let’s Dive into Axygen Microplate 384 Well Pk10 PCR384LC480W
The Axygen Microplate 384 Well Pk10 PCR384LC480W is a specialized consumable designed for high-throughput Polymerase Chain Reaction (PCR) applications, particularly within the Roche 480 Light Cycler. Manufactured by Axygen, these microplates boast a 384-well format, optimized for efficient thermal transfer and precise data acquisition. Each unit contains 10 white-colored plates along with sealing film, packaged in a case of 5 units.
My journey with microplates began over a decade ago, during my early research days focusing on rapid pathogen detection. We struggled with inconsistent results due to poor sealing and well-to-well contamination in our previous plates. We needed a reliable, high-throughput solution compatible with our Roche system.
Upon unboxing the Axygen Microplate 384 Well Pk10 PCR384LC480W, the first thing I noticed was the rigid construction and the bright white color, which is crucial for optimal fluorescence detection. The sealing film appeared robust and well-designed for easy application. The overall build quality exuded a sense of precision, instilling confidence in its performance.
Compared to other microplates I’ve used from brands like Bio-Rad and Eppendorf, the Axygen plates felt noticeably more durable. While the other brands are good, the Axygen plates seemed specifically designed for the Roche Light Cycler, ensuring optimal fit and thermal conductivity. That precise fit was crucial for our lab.
My initial impression was one of cautious optimism. The Axygen Microplate 384 Well Pk10 PCR384LC480W looked and felt like a high-quality product that could potentially solve our contamination and data variability issues. I was eager to put it through its paces.
Real-World Testing: Putting Axygen Microplate 384 Well Pk10 PCR384LC480W to the Test
First Use Experience
Our first major test involved a high-throughput screening experiment for novel antimicrobial compounds. We ran several Axygen Microplate 384 Well Pk10 PCR384LC480W plates in our Roche 480 Light Cycler, processing hundreds of samples. The sealing film was surprisingly easy to apply consistently, which eliminated a major source of error in previous experiments.
The plates performed flawlessly across various temperature cycles. Even with demanding protocols involving rapid temperature changes, the plates maintained their integrity. We noted no warping or deformation, which ensured consistent thermal contact with the instrument’s heating block.
Using the Axygen Microplate 384 Well Pk10 PCR384LC480W was refreshingly straightforward. The 384-well format significantly increased our throughput compared to traditional 96-well plates. We immediately saw a substantial reduction in both time and labor.
Initially, we worried about potential well-to-well contamination, a constant issue in previous high-throughput assays. To our delight, the sealing film provided an excellent barrier, completely preventing any cross-contamination. The consistent data we obtained was a welcome surprise.
Extended Use & Reliability
After several weeks of continuous use, the Axygen Microplate 384 Well Pk10 PCR384LC480W consistently delivered reliable and reproducible results. The plates exhibited impressive durability. Even after repeated handling and incubation steps, they showed no signs of physical degradation.
We rigorously tracked the performance of the plates, focusing on data consistency and the absence of contamination. We found minimal variability across different plates and batches. This translated into more accurate and reliable data.
Cleaning and maintenance were minimal, mainly involving wiping down the exterior with a standard laboratory disinfectant. The robust construction and chemically resistant materials ensured that the plates could withstand repeated cleaning without damage. This was important in our workflow.
Compared to our previous microplate experiences, the Axygen Microplate 384 Well Pk10 PCR384LC480W drastically outperformed our expectations. The consistency, durability, and ease of use significantly improved our experimental workflow and the overall quality of our research. It was a definite upgrade.
Breaking Down the Features of Axygen Microplate 384 Well Pk10 PCR384LC480W
Specifications
- Microplate Type: 384-well PCR microplate. This high-density format is ideal for maximizing throughput.
- Compatibility: Designed for use with Roche 480 Light Cycler. This ensures optimal fit and performance within the instrument.
- Color: White. White color enhances fluorescence signal detection, crucial for accurate PCR quantification.
- Packaging: 10 plates per unit, 5 units per case. Provides a convenient supply for high-volume labs.
- Includes: Sealing film. The included sealing film prevents well-to-well contamination and evaporation during PCR.
- Supplier Number: PCR384LC480W. This ensures you order the correct specific product.
These specifications are crucial for obtaining reliable and accurate PCR results. The compatibility with the Roche 480 Light Cycler ensures proper fit and thermal transfer, while the white color maximizes fluorescence signal. The included sealing film is essential for preventing contamination and evaporation, both of which can lead to inaccurate results.
Performance & Functionality
The Axygen Microplate 384 Well Pk10 PCR384LC480W excels in its primary function: providing a reliable and efficient platform for high-throughput PCR. It delivers consistent thermal transfer and prevents contamination, leading to accurate and reproducible results. The sealing film application is easy, saving time.
The main strength lies in its compatibility with the Roche 480 Light Cycler and the effective sealing film. These features minimize variability and prevent contamination. The only potential area for improvement is the absence of any well labeling on the plate itself; however, this is a minor inconvenience in practice.
Overall, the Axygen Microplate 384 Well Pk10 PCR384LC480W met and exceeded our expectations. It delivered on its promises of high throughput, reliability, and ease of use. We had few issues.
Design & Ergonomics
The Axygen Microplate 384 Well Pk10 PCR384LC480W features a robust and ergonomic design. The plates are made from durable, chemically resistant materials.
The weight is appropriate for easy handling and automated systems. The design is user-friendly, requiring minimal training for effective use.
Durability & Maintenance
The Axygen Microplate 384 Well Pk10 PCR384LC480W is built to last. With proper handling and storage, it can withstand repeated use and standard laboratory conditions.
Maintenance is straightforward. Regular wiping with a laboratory disinfectant is usually sufficient to keep the plates clean and ready for use.
Accessories and Customization Options
The primary accessory included is the sealing film, which is integral to the plate’s performance. While there are no specific customization options for the plates themselves, they are compatible with standard liquid handling robots and automated PCR systems.
The plates are designed to integrate seamlessly with various laboratory workflows. This is a significant advantage for labs with high-throughput needs.
Pros and Cons of Axygen Microplate 384 Well Pk10 PCR384LC480W
Pros
- Exceptional Compatibility: Specifically designed for Roche 480 Light Cycler for optimal performance.
- High-Throughput: 384-well format significantly increases experimental throughput.
- Reliable Sealing: Included sealing film effectively prevents contamination and evaporation.
- Durable Construction: Made from chemically resistant materials for long-lasting use.
- Enhanced Fluorescence: White color maximizes signal detection for accurate PCR quantification.
Cons
- Absence of well labeling on the plate itself can be slightly inconvenient.
- Relatively high cost compared to some generic microplates.
Who Should Buy Axygen Microplate 384 Well Pk10 PCR384LC480W?
The Axygen Microplate 384 Well Pk10 PCR384LC480W is perfect for molecular biology labs, research institutions, and diagnostic facilities that require high-throughput PCR analysis using the Roche 480 Light Cycler. It is also suited for researchers conducting drug discovery, genomics, and proteomics studies.
This product might not be the best choice for labs with limited budgets or those that do not require high-throughput PCR. Also, it is not suitable for instruments other than the Roche 480 Light Cycler.
A must-have accessory would be an automated plate sealer to ensure consistent and reliable sealing of the plates.
Conclusion on Axygen Microplate 384 Well Pk10 PCR384LC480W
The Axygen Microplate 384 Well Pk10 PCR384LC480W is a high-quality, reliable, and efficient consumable for high-throughput PCR applications using the Roche 480 Light Cycler. It’s a significant investment for research labs. The superior performance and reliability justify the price.
I wholeheartedly recommend this product. The Axygen Microplate 384 Well Pk10 PCR384LC480W provides researchers with a powerful tool. It streamlines their workflow. It enables them to generate high-quality, reproducible data. Invest in your science, and invest in Axygen.