Let’s Dive into Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates
The Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates from Axygen Scientific are designed for high-throughput screening, combinatorial chemistry, and other applications where consistent well geometry is critical. These plates promise reliable performance in demanding applications, thanks to their construction from 99.9% pure virgin polypropylene. Priced at $319.99, they aim to balance cost with high-quality construction and RNase-, DNase-, and pyrogen-free certification.
My background is in developing field-deployable diagnostic tools, and consistent, contamination-free reagents are paramount. I was looking for a reliable plate for preparing and storing critical assay components that could also withstand the rigors of transport and varying environmental conditions without compromising sample integrity. The advertised chemical resistance and tight seal capabilities of the Axygen plates caught my attention.
Upon unboxing, the plates felt robust and well-made. The blue color offers a visual cue for easy identification amongst a sea of clear labware. Compared to standard polystyrene plates, these polypropylene plates felt noticeably more durable, with a reassuring rigidity. I was also evaluating Corning Costar 96-well plates and Greiner Bio-One plates, but the Axygen plates’ claim of being manufactured from 99.9% pure virgin polypropylene and their V-bottom design swayed my decision due to its suitability for complete sample retrieval.
Initially, I was cautiously optimistic. The price is certainly a factor, but if the Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates deliver on their promises of purity, consistency, and durability, the investment would be well worth it.
Real-World Testing: Putting Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates to the Test
First Use Experience
My initial testing involved preparing serial dilutions of a fluorescent protein standard for a novel immunoassay. These dilutions were critical for calibrating our detection system in the field. The plates were used in a controlled laboratory setting as well as transported in a portable cooler to a simulated field site with fluctuating temperatures.
The plates were easy to use, fitting seamlessly into our automated liquid handling system. The V-bottom design facilitated complete removal of liquid, crucial for accurate serial dilutions. I encountered no leakage or cross-contamination, even during rigorous shaking and transportation simulations.
Extended Use & Reliability
After several weeks of consistent use, the Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates continue to perform flawlessly. The plates show no signs of wear and tear, even after repeated autoclaving cycles. Cleaning is straightforward. A simple wash with deionized water and ethanol is sufficient to remove any residue.
Compared to previous plates I’ve used from other manufacturers, the Axygen plates exhibit superior chemical resistance, showing no signs of degradation after prolonged exposure to organic solvents and concentrated salt solutions. The consistent well volume and shape also translate to more reproducible results, a significant improvement over some cheaper alternatives.
Breaking Down the Features of Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates
Specifications
The Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates boast several key specifications. The color is blue, allowing for easy visual identification. They are non-sterile, making them suitable for a range of applications beyond sterile cell culture.
The Axygen No. is P-96-450-V-B, essential for consistent reordering. The working well volume for V-Bottom Plates is 480 µL, providing ample space for most assay volumes. The working well volume for Round Bottom Plates is 500 µL.
These specifications are important because they ensure consistency and reliability in experimental results. The specific well volume is critical for accurate dilutions and reagent mixing. The V-bottom design facilitates complete sample recovery, minimizing waste and maximizing assay sensitivity.
Performance & Functionality
The Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates perform exceptionally well. The V-bottom design enables efficient mixing and complete aspiration of liquids. The plates are compatible with standard plate readers and robotic handling systems, streamlining workflows.
The primary strength is their ability to maintain sample integrity under various conditions. A minor weakness is the higher cost compared to standard polystyrene plates. Overall, the Axygen plates meet and exceed my expectations.
Design & Ergonomics
The build quality is excellent. The plates are manufactured from 99.9% pure virgin polypropylene, providing exceptional chemical resistance and durability. The plates are easy to handle. They fit securely in plate holders and automated systems.
The blue color is a welcome addition, making it easier to differentiate these plates from others in the lab. The V-bottom shape aids in efficient sample retrieval.
Durability & Maintenance
These plates are designed to last. They are resistant to cracking, warping, and chemical degradation. Maintenance is minimal. Simple washing and autoclaving are sufficient for sterilization.
Accessories and Customization Options
The plates do not come with specific accessories, but Axygen Scientific offers a range of compatible sealing mats. These mats are chemically resistant and provide a secure seal to prevent evaporation and contamination. The plates are compatible with standard microplate readers and automation systems.
Pros and Cons of Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates
Pros
- Superior chemical resistance due to the use of 99.9% pure virgin polypropylene.
- V-bottom design enables complete sample recovery and efficient mixing.
- Consistent well volume and shape lead to more reproducible experimental results.
- Durable construction withstands repeated autoclaving cycles and harsh chemicals.
- Blue color aids in easy visual identification and organization.
Cons
- Higher cost compared to standard polystyrene assay plates.
- Non-sterile, requiring sterilization for specific cell culture applications.
Who Should Buy Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates?
The Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates are perfect for researchers and scientists working in high-throughput screening, combinatorial chemistry, genomics, proteomics, and drug discovery. Those who require consistent, contamination-free results and reliable sample handling will greatly benefit. They are also ideal for applications involving harsh chemicals or extreme temperatures.
Researchers on a tight budget and who do not require the specific features of a V-bottom or the high chemical resistance might consider cheaper polystyrene alternatives. Those needing sterile plates directly out of the packaging should look for pre-sterilized options. A must-have accessory is a compatible sealing mat to prevent evaporation and contamination during incubation and storage.
Conclusion on Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates
In summary, the Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates are a high-quality and reliable choice for demanding laboratory applications. Their superior chemical resistance, consistent well geometry, and durable construction justify the higher price point. I would wholeheartedly recommend these plates to anyone seeking to improve the accuracy and reproducibility of their experiments.
If you are serious about your research and require top-notch materials, invest in the Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-B V-Bottom Plates. You won’t be disappointed.