Let’s Dive into Corning BioCoat Cellware, Collagen Type I, 356408 Multiwell Plates 24-Well, Clear, Case of 50
The Corning BioCoat Cellware, Collagen Type I, 356408 Multiwell Plates 24-Well, Clear, Case of 50 are a critical piece of equipment for any lab working with cell cultures. These plates, manufactured by Corning, provide a standardized and reliable platform for cell attachment, proliferation, and differentiation. The Collagen Type I coating mimics the natural extracellular matrix, promoting a more in vivo-like environment for cells, thereby enhancing their behavior and response.
My research team was struggling with consistent cell attachment when working with primary hepatocytes. We needed a reliable and consistent surface to improve our data reproducibility.
The Corning BioCoat Cellware, Collagen Type I, 356408 Multiwell Plates 24-Well, Clear, Case of 50 arrived in a sturdy cardboard box, well-protected against damage during shipping. Opening the case revealed individually wrapped stacks of plates, ensuring sterility. The plates themselves were clear and free from any visible defects.
Before choosing the Corning BioCoat Cellware, we considered standard tissue culture-treated plates and plates with other coatings like Poly-L-Lysine. However, the literature suggested that Collagen Type I would provide the best results for our specific cell type. We also valued the Corning brand reputation for consistency and quality.
I was cautiously optimistic. We had high hopes that these plates would solve our cell attachment issues, but also recognized that cell culture can be finicky, and results aren’t always guaranteed.
Real-World Testing: Putting Corning BioCoat Cellware, Collagen Type I, 356408 Multiwell Plates 24-Well, Clear, Case of 50 to the Test
First Use Experience
The plates were tested in our cell culture lab, specifically in a sterile biosafety cabinet. We cultured primary hepatocytes on the plates using standard cell culture protocols. The plates were incubated at 37°C with 5% CO2.
The humidity in our incubator fluctuated significantly on the first day. However, the plate integrity remained consistent, indicating resilience. We observed remarkably improved cell attachment compared to our previous attempts on uncoated plates.
The plates were incredibly easy to use, requiring no special preparation beyond standard sterile handling. The cells adhered quickly and evenly across the wells.
We did find that the cells initially exhibited a slightly different morphology compared to what we were used to, but this normalized within 24 hours. This was likely due to the enhanced cell-matrix interaction.
Extended Use & Reliability
After several weeks of using the Corning BioCoat Cellware, we’ve consistently observed excellent cell attachment and viability. The plates have become an indispensable tool in our lab.
The plates have shown no signs of degradation or coating detachment, even with repeated media changes. The Collagen Type I coating remains stable and functional.
Maintenance is straightforward: standard aspiration and media replacement procedures are sufficient. No special cleaning or handling is required.
Compared to our previous experience with uncoated plates, the Corning BioCoat Cellware has dramatically improved our experimental outcomes. We’ve seen increased cell proliferation rates, improved cell differentiation, and reduced cell detachment during experiments.
Breaking Down the Features of Corning BioCoat Cellware, Collagen Type I, 356408 Multiwell Plates 24-Well, Clear, Case of 50
Specifications
- Product Name: Corning BioCoat Cellware, Collagen Type I, 356408 Multiwell Plates 24-Well, Clear, Case of 50
- Manufacturer: Corning
- Well Format: 24-well
- Plate Material: Clear polystyrene
- Coating: Collagen Type I
- Sterility: Sterile
- Nonpyrogenic: Yes
- Packaging: Case of 50 plates
- Shelf Life: At least three months from date of shipment when stored at 2 to 8°C (36 to 46°F)
The 24-well format provides a balance between throughput and cell culture volume, suitable for many experiments. The clear polystyrene allows for easy visualization of cells under a microscope.
The Collagen Type I coating is the most crucial specification, as it dictates the cell attachment and behavior. Sterility and being nonpyrogenic are essential for avoiding contamination and ensuring reliable results.
Performance & Functionality
The Corning BioCoat Cellware excels at promoting cell attachment and proliferation. The cells exhibit a more natural morphology and respond better to stimuli.
The primary strength is the consistent and reliable Collagen Type I coating. A minor weakness is the cost, as coated plates are more expensive than uncoated ones.
The plates exceeded our expectations in terms of improving cell attachment and overall culture quality. Our experimental data has become more reproducible and reliable.
Design & Ergonomics
The plates are well-designed and easy to handle. The standard 24-well format is compatible with automated liquid handling systems.
The clear polystyrene material allows for excellent visibility under a microscope. The plates are lightweight and comfortable to handle during extended experiments.
Durability & Maintenance
With proper handling and storage, the plates should last for at least the specified shelf life. The Collagen Type I coating is robust and does not easily detach.
Maintenance is minimal, requiring only standard cell culture techniques. The plates are disposable, eliminating the need for cleaning.
Accessories and Customization Options
The plates do not come with any additional accessories. However, they are compatible with standard cell culture media, supplements, and reagents.
No direct customization is available, but users can select different coatings or well formats based on their specific needs. For example, Corning offers similar plates with Poly-L-Lysine coatings.
Pros and Cons of Corning BioCoat Cellware, Collagen Type I, 356408 Multiwell Plates 24-Well, Clear, Case of 50
Pros
- Superior cell attachment compared to uncoated plates.
- Consistent and reliable Collagen Type I coating.
- Improved cell proliferation and differentiation.
- Enhanced experimental reproducibility.
- Easy to use with standard cell culture protocols.
Cons
- Higher cost compared to uncoated plates.
- Shelf life requires careful inventory management.
Who Should Buy Corning BioCoat Cellware, Collagen Type I, 356408 Multiwell Plates 24-Well, Clear, Case of 50?
These plates are perfect for researchers working with primary cells, stem cells, or transfected mammalian cells. Labs that require high cell attachment and consistent cell culture conditions will benefit the most.
Labs on a very tight budget or those working with cell lines that readily attach to uncoated surfaces might be able to skip this product. However, the improved cell behavior often justifies the added cost.
Consider using high-quality cell culture media and supplements to maximize the benefits of the Collagen Type I coating. A reliable CO2 incubator is also essential for optimal cell growth.
Conclusion on Corning BioCoat Cellware, Collagen Type I, 356408 Multiwell Plates 24-Well, Clear, Case of 50
The Corning BioCoat Cellware, Collagen Type I, 356408 Multiwell Plates 24-Well, Clear, Case of 50 are a valuable investment for any cell culture lab seeking to improve cell attachment and experimental reproducibility. The Collagen Type I coating provides a biologically relevant surface that promotes healthy cell growth and function.
The price is justified by the improved data quality and reduced experimental variability. For serious cell culture research, skimping on quality is a mistake.
I would highly recommend these plates to any researcher working with cells that require enhanced attachment. The Corning BioCoat Cellware has become an indispensable tool in our lab.
If your cells are struggling to attach and thrive, invest in the Corning BioCoat Cellware, Collagen Type I, 356408 Multiwell Plates 24-Well, Clear, Case of 50 – your experiments (and your sanity) will thank you.