Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates Review

Let’s Dive into Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates

The Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates might not be the typical gear I usually review, swapping bullets for bacteria, but precision and reliability remain paramount. These plates, manufactured by Axygen Scientific, are a cornerstone in biochemical testing, boasting 96 wells of virgin polypropylene. Their V-bottom design is specifically engineered for optimal sample recovery, a critical detail in sensitive assays.

My introduction to these plates came during a particularly demanding project involving environmental DNA analysis. We needed a reliable, high-throughput solution for processing a large number of samples, and contamination was simply not an option. The Axygen plates promised purity and consistency, qualities that immediately piqued my interest.

Unboxing revealed a neatly packaged set of yellow plates, their construction feeling robust despite their delicate purpose. The plates felt uniform and sturdy. The included mats seemed like a practical addition, hinting at the product’s attention to detail, promising a secure seal.

Compared to standard flat-bottom plates we had previously used, the V-bottom design was a clear advantage for concentrating samples. Disposable polystyrene plates were also considered, but the polypropylene of the Axygen plates offered superior chemical resistance and reduced risk of leaching. The Axygen plates offered more reliable performance and were better suited for long-term storage.

My first impression was cautiously optimistic. The plates looked the part, exuding a sense of quality and purpose. It was time to put them to work and see if they could live up to the hype and tackle our challenging experimental design.


Real-World Testing: Putting Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates to the Test

First Use Experience

The initial testing involved culturing various environmental bacterial strains under different nutrient conditions. The plates were used in a controlled laboratory setting, where temperature and humidity were carefully maintained. The plates were loaded with samples in a sterile environment.

The Axygen plates performed flawlessly, with no evidence of cross-contamination or leakage between wells. The V-bottom shape greatly facilitated the collection of bacterial pellets after centrifugation, a significant improvement over our previous flat-bottom plates. Pipetting solutions into each well was also straightforward.

I didn’t anticipate the plates’ compatibility with our automated liquid handling system. This immediately streamlined the workflow, reducing manual handling and minimizing potential errors. After first use, the plates instilled confidence in their reliability.

Extended Use & Reliability

After several weeks of continuous use, the Axygen plates continued to perform consistently. We’ve used them for everything from enzyme assays to DNA quantification, and they have proven to be reliable in each application. The plates held up extremely well, even after repeated cycles of heating, cooling, and centrifugation.

We have observed no signs of degradation or warping, even after prolonged exposure to various chemicals and solvents. Cleaning is a breeze, as the polypropylene material is resistant to staining and easily wipes clean with standard laboratory detergents. The plates proved easier to clean than polystyrene alternatives.

The Axygen plates have definitely outperformed the generic plates we were using previously. The superior construction and thoughtful design have resulted in more consistent and reliable experimental results, minimizing wasted time and resources. The switch to Axygen plates has increased our confidence in our research.

Breaking Down the Features of Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates

Specifications

  • The Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates feature a 96-well format, conforming to standard microplate dimensions for compatibility with automated systems. This allows for high-throughput processing of samples.

  • Each well has a V-bottom design, optimized for complete sample recovery and concentration. The V-bottom minimizes dead volume, ensuring even small volumes can be easily aspirated.

  • The plates are constructed from 99.9% pure virgin polypropylene, ensuring exceptional chemical resistance and minimal risk of contamination. This ensures the integrity of sensitive experiments.

  • They are certified RNase-, DNase-, and pyrogen-free, guaranteeing the absence of any interfering biological contaminants. These plates meet stringent quality control standards.

  • Each plate has a Working Well Volume of 480 µL. This volume provides ample space for various assay types while minimizing spill-over risks.

  • The plates are Yellow in color and are Nonsterile, allowing them to be customized for specific experimental needs. The yellow color offers better contrast for sample identification.

  • The plates have the Axygen No.: P-96-450-V-Y. This number ensures consistent product identification and ordering.

These specifications are critical because they directly impact the accuracy and reliability of biochemical assays. The pure polypropylene construction ensures the integrity of sensitive samples, while the V-bottom design optimizes sample recovery, minimizing waste and improving overall efficiency. The product is free of contaminants such as RNase and DNase.

Performance & Functionality

The Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates excel at their primary function: providing a reliable and contamination-free platform for biochemical assays. Their uniform construction and precise dimensions ensure consistent results across all 96 wells. The uniform construction assures reliable results.

The V-bottom design significantly enhances sample recovery, particularly when working with small volumes or dilute solutions. This minimizes waste and maximizes the sensitivity of downstream analyses. The V-bottom design is an efficient design.

However, the non-sterile nature of the plates may require additional preparation steps for certain applications. Sterilization by autoclaving or irradiation may be necessary. The plates’ non-sterile nature is a compromise.

Overall, the plates meet and exceed expectations for performance and functionality. Their reliability and consistency make them an indispensable tool for high-throughput biochemical research. These plates are a robust and trustworthy product.

Design & Ergonomics

The build quality is excellent, with the polypropylene material providing a sturdy and durable platform. The plates are lightweight yet resist warping or cracking, even under stress. These plates are resistant to damage.

The plates are user-friendly, with standard dimensions that fit seamlessly into most automated liquid handling systems. No learning curve is required; simply load and go. These plates are an easy-to-use product.

Durability & Maintenance

The Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates are designed for long-term use, capable of withstanding repeated cycles of heating, cooling, and chemical exposure. With proper care, they should last for years in a laboratory setting. The plates are durable and able to withstand many uses.

Maintenance is straightforward, with the smooth polypropylene surface easily cleaned with standard laboratory detergents. Avoid abrasive cleaners, which could scratch the surface and compromise the integrity of the wells. Harsh chemicals should also be avoided.

Accessories and Customization Options

The Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates come standard with sealing mats designed to prevent evaporation and cross-contamination. The mats provide a secure and reliable seal.

While the plates themselves are not extensively customizable, they are compatible with a wide range of standard laboratory accessories, such as barcode labels and plate readers. The plates fit into most existing laboratory workflows. The ability to integrate into automated systems makes them a versatile tool for any laboratory.

Pros and Cons of Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates

Pros

  • Exceptional purity: Constructed from 99.9% virgin polypropylene, ensuring minimal contamination. This provides maximum purity for scientific experiments.
  • Optimized Sample Recovery: V-bottom design facilitates complete sample recovery, minimizing waste. The shape of the wells provides full recovery of materials.
  • High Chemical Resistance: Polypropylene construction resists degradation from a wide range of chemicals and solvents. This chemical resistance ensures the integrity of sensitive assays.
  • Standard Dimensions: Compatible with automated liquid handling systems and plate readers. The standard dimensions are compatible with other equipment.
  • Certified Free of Contaminants: RNase-, DNase-, and pyrogen-free, guaranteeing the absence of interfering biological contaminants. The product is manufactured to be free of contaminants.

Cons

  • Non-Sterile: Requires additional sterilization steps for certain applications. Sterilizing is an extra step that might not be needed in a sterile product.
  • Cost: Higher price point compared to generic assay plates. The value is worth it, but there are other cheaper plates out there.


Who Should Buy Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates?

The Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates are perfect for researchers, scientists, and technicians involved in high-throughput biochemical assays, molecular biology experiments, and drug discovery. Anyone who needs reliable, contamination-free results will benefit. Labs conducting environmental testing will benefit from these plates.

Individuals or labs on a very tight budget may want to explore cheaper alternatives, but be aware that compromising on quality can lead to unreliable results and wasted resources. These plates are worth the investment for reliable experiments.

Must-have accessories include a reliable sealing mat, appropriate sterilization equipment (if required), and compatible pipettes and liquid handling systems. Consider automated systems for high-throughput experiments.

Conclusion on Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates

The Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates are a high-quality, reliable, and consistent choice for demanding biochemical assays. While the price point is higher than generic alternatives, the superior performance and reduced risk of contamination justify the investment. The plates are an investment in reliability.

I would personally recommend these plates to any researcher or technician who values accuracy and efficiency in their work. Their robust construction, optimized design, and guaranteed purity make them an indispensable tool for any modern laboratory. If you’re serious about your science, invest in the Axygen 96-Well Assay Plates, Axygen Scientific P-96-450-V-Y V-Bottom Plates.

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