Let’s Dive into Eppendorf Pipette Res Plus Fixed 250ul 3121000104
The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 is a single-channel, fixed-volume pipette designed for precision liquid handling in laboratory settings. Eppendorf is renowned for its commitment to quality and ergonomic design, promising accuracy and comfort for users. The pipette boasts a spring-loaded tip cone, aiming for optimal sealing and reduced force during tip ejection.
My need for this pipette stemmed from a project involving precise aliquoting of solutions for enzyme assays. Manual pipetting with adjustable pipettes introduced variability that significantly affected the reproducibility of my experiments. I needed a solution that offered consistent volume dispensing and minimized user-induced errors.
Upon unboxing the Eppendorf Pipette Res Plus Fixed 250ul 3121000104, I was immediately struck by its solid construction. The pipette felt well-balanced in my hand, and the blue coloring, specified by Eppendorf, was a welcome touch. Initial impressions suggested a high-quality instrument built to last.
Compared to my existing collection of adjustable pipettes from various manufacturers, the Eppendorf stood out for its simplicity and defined purpose. While adjustable pipettes offer versatility, I found the fixed-volume design of the Eppendorf Pipette Res Plus Fixed 250ul 3121000104 provided a level of confidence in volume accuracy that adjustable pipettes lacked. It addresses a niche need that the others couldn’t fully satisfy.
My first impression was one of cautious optimism mixed with relief. I was excited to finally have a pipette specifically calibrated for a critical volume in my work. The promise of reduced operator fatigue and increased reproducibility was also very appealing, providing a measure of relief after struggling with inaccurate results previously.
Real-World Testing: Putting Eppendorf Pipette Res Plus Fixed 250ul 3121000104 to the Test
First Use Experience
My initial testing ground for the Eppendorf Pipette Res Plus Fixed 250ul 3121000104 was in my research laboratory, where I routinely perform enzyme kinetic assays. I used it to dispense precise volumes of substrate into microplate wells. The laboratory environment is generally climate-controlled, but slight temperature and humidity fluctuations are inevitable.
The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 handled these variations admirably. Its spring-loaded tip cone consistently provided a tight seal, preventing any leakage or air gaps that could compromise accuracy. I found the pipette easy to use from the outset.
The smooth plunger action and comfortable grip minimized fatigue during prolonged pipetting sessions. A slight learning curve involved mastering the proper pipetting technique, including the correct immersion depth and aspiration/dispensing speed. Initially, I noticed a very slight “hesitation” in the piston action, but this disappeared after a few uses.
Extended Use & Reliability
After several months of consistent use, the Eppendorf Pipette Res Plus Fixed 250ul 3121000104 continues to perform reliably. I have experienced no significant performance degradation. The pipette shows minimal signs of wear and tear.
The sturdy plastic housing resists scratches and chemical stains. Maintenance is relatively straightforward, primarily involving wiping down the exterior with a lint-free cloth and occasionally cleaning the tip cone with isopropyl alcohol. Compared to cheaper adjustable pipettes I’ve used in the past, the Eppendorf offers a superior level of precision and consistency over time.
It has noticeably improved the reproducibility of my enzyme assays. The fixed volume and reliable tip seal have eliminated many sources of error. The Eppendorf has exceeded my expectations in terms of both performance and durability, and become an indispensable tool in the lab.
Breaking Down the Features of Eppendorf Pipette Res Plus Fixed 250ul 3121000104
Specifications
The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 is a single-channel pipette designed for dispensing a fixed volume of 250 microliters (µl). Its defining feature is the fixed volume, ensuring consistent dispensing without the risk of accidental volume adjustments. The pipette body is constructed from durable, chemically resistant plastic.
The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 also features a spring-loaded tip cone. This feature provides optimal tip sealing, reducing the force required to attach and eject tips. This matters because the spring-loaded tip cone improves dispensing consistency. It also reduces the risk of repetitive strain injuries for users performing high-throughput pipetting.
Performance & Functionality
The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 excels at its primary function: dispensing accurate and reproducible 250 µl volumes. I have verified its accuracy using gravimetric methods and spectrophotometric assays. My results confirm that it consistently delivers volumes within the specified accuracy range.
One of its greatest strengths is its ease of use. The smooth plunger action and ergonomic design make it comfortable to use for extended periods. A potential weakness is its lack of volume adjustability; this makes it unsuitable for applications requiring variable dispensing volumes. The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 has undeniably met my expectations.
Design & Ergonomics
The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 boasts a well-thought-out design that prioritizes ergonomics. The pipette feels balanced and comfortable in the hand, even during long pipetting sessions. The materials used are high-quality and chemically resistant.
The smooth plunger action and intuitive design minimize user fatigue. The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 has a minimal learning curve. The single-button operation simplifies the pipetting process.
Durability & Maintenance
The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 is built to withstand the rigors of daily laboratory use. Its robust construction suggests it will provide years of reliable service. Maintaining the pipette is straightforward.
Regular cleaning with appropriate solvents is sufficient to prevent contamination and ensure optimal performance. Disassembly for in-depth cleaning or repairs requires specialized tools and expertise.
Accessories and Customization Options
The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 comes with a basic calibration certificate. This certificate confirms its accuracy and traceability. Customization options are limited due to its fixed-volume design.
The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 is compatible with standard 200-300 µl pipette tips from various brands. This broad compatibility provides flexibility in tip selection. The spring-loaded tip cone contributes to enhanced tip sealing, irrespective of the brand used.
Pros and Cons of Eppendorf Pipette Res Plus Fixed 250ul 3121000104
Pros
- Exceptional accuracy and reproducibility: Delivers consistent 250 µl volumes, minimizing experimental error.
- Ergonomic design: Comfortable to use for extended periods, reducing user fatigue.
- Durable construction: Built to withstand the rigors of daily laboratory use.
- Spring-loaded tip cone: Ensures optimal tip sealing and minimizes force required for tip ejection.
- Simple and intuitive operation: Easy to learn and use, even for inexperienced users.
Cons
- High Price: At $699.99, this is a significantly more expensive pipette than adjustable volume models.
- Fixed volume limitation: Unsuitable for applications requiring variable dispensing volumes.
Who Should Buy Eppendorf Pipette Res Plus Fixed 250ul 3121000104?
The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 is perfect for researchers and laboratory technicians who require highly accurate and reproducible dispensing of 250 µl volumes. It is also a great fit for clinical laboratories and pharmaceutical companies. Its precise design and execution make it ideal for repeatable testing procedures.
This pipette might not be suitable for users who need a general-purpose pipette capable of dispensing a wide range of volumes. It is highly specialized and is best suited for those whose main objective is dispensing one set volume with extreme accuracy.
A must-have accessory is a high-quality supply of compatible pipette tips from a reputable manufacturer. Regularly verifying the pipette’s calibration is also recommended, which may require specialized equipment and expertise.
Conclusion on Eppendorf Pipette Res Plus Fixed 250ul 3121000104
The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 is a specialized instrument that excels at its intended purpose: delivering highly accurate and reproducible 250 µl volumes. Its ergonomic design and durable construction make it a valuable asset for any laboratory requiring precise liquid handling. Despite its relatively high price of $699.99, the benefits in terms of accuracy and reliability justify the investment for those who require it.
I would wholeheartedly recommend this pipette to anyone who prioritizes precision and consistency in their work and dispenses 250 µL volumes regularly. The Eppendorf Pipette Res Plus Fixed 250ul 3121000104 has transformed my enzyme assays. It has significantly improved the reliability of my results. If you’re serious about your science, consider investing in this instrument.