Let’s Dive into DMEM/High: with 4500 mg/L Glucose and 4.0 mM L-Glutamine, without Sodium Pyruvate PK6 SH30022.LS
The DMEM/High: with 4500 mg/L Glucose and 4.0 mM L-Glutamine, without Sodium Pyruvate PK6 SH30022.LS from Hyclone represents a critical component in cell culture, offering a precisely formulated medium for supporting the growth of various mammalian cells. Its high glucose concentration caters to cell lines with high energy demands. The inclusion of L-Glutamine provides an essential amino acid crucial for cell proliferation and metabolism.
My journey to this particular product stemmed from a frustrating period in our lab. We were consistently facing issues with cell viability across several projects. Different media formulations were yielding inconsistent results, and we needed a reliable, standardized solution to eliminate this variable.
Upon receiving the package, I was immediately struck by the professional packaging and secure bottles. Each 1000 ml bottle felt robust and designed to maintain sterility. The clear labeling made it easy to identify the specific formulation and batch number, instilling confidence in the product’s quality control.
We also looked at Gibco’s DMEM and Sigma-Aldrich’s DMEM. The Hyclone product won out based on a combination of price point and availability in the specific formulation needed: high glucose, L-Glutamine included, and importantly, without Sodium Pyruvate for our particular cell lines.
I was initially hopeful, but cautiously optimistic. Cell culture is notoriously finicky, and I’ve learned not to get my hopes up too high until I see tangible results.
Real-World Testing: Putting DMEM/High: with 4500 mg/L Glucose and 4.0 mM L-Glutamine, without Sodium Pyruvate PK6 SH30022.LS to the Test
First Use Experience
Our initial testing involved culturing CHO cells and HEK293 cells, both notoriously sensitive to media variations. We replaced our existing media with the Hyclone DMEM/High following standard protocols. The cells responded remarkably well, showing significantly improved growth rates and viability compared to our previous media.
The medium maintained a stable pH throughout the incubation period, even under slightly varied CO2 concentrations. This is crucial for consistent experimental results. We noticed a subtle difference in the cell morphology, appearing healthier and more robust compared to cells grown in our older, less consistent media.
There were no immediate issues or surprises. The cells took to the new media seamlessly, and we observed a noticeable improvement within the first 24 hours.
Extended Use & Reliability
After several weeks of continuous use, the DMEM/High has consistently delivered excellent results. Cell growth remains robust and predictable. We’ve observed minimal batch-to-batch variation, which is crucial for maintaining experimental integrity.
The media shows no signs of degradation even after being stored in the refrigerator for the recommended period. This confirms the quality of the formulation and packaging. Maintaining the proper storage temperature is key to ensuring the media’s longevity and effectiveness.
Compared to previous media, the Hyclone DMEM/High significantly outperforms in terms of consistency and reliability. We’ve reduced cell death and improved overall experimental reproducibility. This product has genuinely streamlined our cell culture workflows.
Breaking Down the Features of DMEM/High: with 4500 mg/L Glucose and 4.0 mM L-Glutamine, without Sodium Pyruvate PK6 SH30022.LS
Specifications
The DMEM/High: with 4500 mg/L Glucose and 4.0 mM L-Glutamine, without Sodium Pyruvate PK6 SH30022.LS boasts a precise formulation designed to support a wide range of mammalian cell cultures. It contains a high glucose concentration of 4500 mg/L, providing ample energy for rapidly dividing cells. The inclusion of 4.0 mM L-Glutamine supplies a vital amino acid for protein synthesis and cellular metabolism.
Notably, this formulation excludes Sodium Pyruvate. This is important for cell lines that either produce their own pyruvate or are sensitive to exogenous pyruvate supplementation. The media is manufactured by Hyclone, a reputable name in cell culture, and is supplied in a pack of six 1000 ml bottles, ensuring ample supply for consistent experimentation.
These specifications directly impact cell growth, viability, and overall experimental outcomes. The precise formulation ensures optimal nutrient availability, while the absence of Sodium Pyruvate caters to specific cellular needs.
Performance & Functionality
The DMEM/High performs exceptionally well in supporting cell growth and maintaining cell viability. It provides a stable and consistent environment for cell culture. Our experiments showed improved cell proliferation rates and reduced cell death compared to previous media formulations.
One minor area for potential improvement could be the inclusion of a more detailed certificate of analysis (COA) directly on the bottle. While the COA is included in the package, having a concise version readily available on each bottle would streamline record-keeping. Overall, the product exceeded our expectations.
Design & Ergonomics
The Hyclone DMEM/High is packaged in durable, unbreakable PETG bottles. These bottles are easy to handle and store. The clear labeling allows for quick identification of the product and batch number.
The bottles are designed to minimize the risk of contamination during pouring. A larger, ergonomic grip would further enhance the user experience, especially when handling multiple bottles at once.
Durability & Maintenance
The media itself is stable and maintains its quality for an extended period when stored correctly. Proper storage at 2-8°C is crucial. The PETG bottles are robust and resistant to damage, ensuring the media remains sterile and uncontaminated.
The tightly sealed caps prevent leakage and evaporation, preserving the integrity of the formulation. Regular monitoring of the expiration date is essential.
Accessories and Customization Options
The DMEM/High does not require any specific accessories for its use, but is typically supplemented with fetal bovine serum (FBS) and antibiotics/antimycotics. The media is compatible with a wide range of supplements and additives commonly used in cell culture. Researchers can tailor the media to their specific cell line requirements by adding growth factors, hormones, or other desired components. This flexibility is a key advantage.
Pros and Cons of DMEM/High: with 4500 mg/L Glucose and 4.0 mM L-Glutamine, without Sodium Pyruvate PK6 SH30022.LS
Pros
- Excellent cell growth and viability: Consistently delivers robust cell proliferation.
- Stable and consistent formulation: Minimal batch-to-batch variation ensures reliable results.
- Specifically formulated without Sodium Pyruvate: Meets the needs of particular cell lines.
- Durable and secure packaging: PETG bottles prevent leakage and contamination.
- Cost-effective solution: Provides excellent value for its performance.
Cons
- Certificate of Analysis (COA) not printed directly on the bottle.
- Bottle grip could be more ergonomic.
Who Should Buy DMEM/High: with 4500 mg/L Glucose and 4.0 mM L-Glutamine, without Sodium Pyruvate PK6 SH30022.LS?
This media is perfect for researchers and cell culture technicians working with mammalian cell lines that require high glucose concentrations and L-Glutamine, but do not need or tolerate Sodium Pyruvate. It is ideal for those seeking a reliable and consistent media formulation. This product is particularly well-suited for academic and industrial research labs.
Researchers working with cell lines that require Sodium Pyruvate should skip this product. Those on a very tight budget might explore alternative brands. However, the Hyclone DMEM/High offers a superior balance of quality and price.
Must-have accessories include high-quality Fetal Bovine Serum (FBS) and appropriate antibiotics/antimycotics. Ensure proper sterile technique during handling.
Conclusion on DMEM/High: with 4500 mg/L Glucose and 4.0 mM L-Glutamine, without Sodium Pyruvate PK6 SH30022.LS
The DMEM/High: with 4500 mg/L Glucose and 4.0 mM L-Glutamine, without Sodium Pyruvate PK6 SH30022.LS from Hyclone is a top-tier cell culture media that delivers exceptional performance and consistency. Its precisely formulated composition supports robust cell growth. The exclusion of Sodium Pyruvate caters to specific cellular needs.
The price is certainly justified given the improved cell viability and experimental reproducibility we’ve observed. It is a worthwhile investment for any lab seeking reliable cell culture results.
I would absolutely recommend this product to any researcher or cell culture technician looking for a high-quality, dependable media solution. It has significantly improved our cell culture workflows and boosted our experimental success. Give it a try, and see the difference for yourself!