Let’s Dive into BD Purecoat Amine Flask 175 Cs50 356728
The BD Purecoat Amine Flask 175 Cs50 356728, manufactured by BD, is a specialized cell culture flask designed for advanced biological research and applications requiring enhanced cell adhesion. It offers a 175 cm² growth area and comes in a case of 50, promising consistent and reliable performance. I’ve spent years working with various cell culture methods, and the need for reliable and consistent cell adhesion has always been a critical factor in my research.
My hunt for the BD Purecoat Amine Flask 175 Cs50 356728 started when my existing cell culture methods proved inconsistent for a new line of particularly finicky cells. Standard TC-treated flasks weren’t cutting it; the cells were detaching too easily, leading to unreliable data and wasted resources. I needed a surface that would promote stronger, more reliable cell attachment.
Upon unboxing, the flasks presented a clean, sterile, and professional appearance. The build quality felt sturdy and reliable, and the packaging ensured the flasks arrived undamaged. The weight felt appropriate for the size, suggesting a robust construction that could withstand standard laboratory handling.
Compared to standard TC-treated flasks and some competing amine-coated options, the BD Purecoat Amine Flask 175 Cs50 356728 stood out due to BD‘s reputation for high-quality cell culture products and the specified surface area. I had previously used BD‘s other cell culture products with great success, which significantly influenced my decision to try these flasks. I was particularly interested in how the amine coating would affect cell adhesion compared to poly-l-lysine coated flasks, which I had also been considering.
My first impression was a mix of excitement and cautious optimism. The price tag of $769.99 is substantial, so I hoped the performance would justify the investment. I was eager to see if the BD Purecoat Amine Flask 175 Cs50 356728 would truly deliver superior cell adhesion and consistent results.
Real-World Testing: Putting BD Purecoat Amine Flask 175 Cs50 356728 to the Test
First Use Experience
I tested the BD Purecoat Amine Flask 175 Cs50 356728 in my cell culture lab, specifically for culturing primary neurons, which are notoriously difficult to adhere and maintain in vitro. The testing involved seeding the neurons into the flasks under standard cell culture conditions (37°C, 5% CO2) and monitoring their adhesion and growth over several days. I specifically looked for the number of cells attached after 24 hours, their morphology, and their ability to form healthy neuronal networks.
The flasks performed remarkably well even in slightly suboptimal conditions. I deliberately introduced minor variations in temperature and CO2 levels to simulate real-world lab fluctuations. The BD Purecoat Amine Flask 175 Cs50 356728 still facilitated better cell adhesion and growth compared to control flasks.
Using these flasks was incredibly straightforward. Standard cell culture protocols applied seamlessly. No special preparations or modifications were required, making integration into my existing workflow hassle-free.
Initially, I was pleasantly surprised by the significantly enhanced cell adhesion observed within the first 24 hours. The neurons attached more rapidly and displayed a healthier morphology compared to those grown in standard flasks. This initial success boosted my confidence in the flask’s performance.
Extended Use & Reliability
After several weeks of continuous use, the BD Purecoat Amine Flask 175 Cs50 356728 consistently delivered excellent results. Cell cultures exhibited robust growth and maintained high viability over extended periods. The amine coating remained effective throughout the entire experiment, showing no signs of degradation.
The flasks have shown no signs of wear and tear during routine handling and incubation. The plastic material remained robust and transparent, allowing for clear visualization of the cell cultures. They have proven durable under standard laboratory conditions.
Maintaining the flasks is as simple as cleaning standard cell culture flasks. Regular rinsing with sterile water and sterilization using standard autoclaving protocols have kept the flasks in optimal condition. The amine coating has proven resistant to degradation during the cleaning process.
Compared to my previous experiences with standard TC-treated flasks, the BD Purecoat Amine Flask 175 Cs50 356728 significantly outperformed them in terms of cell adhesion, growth rate, and culture longevity. The consistency and reliability of the results have been a game-changer for my research. I no longer have to worry about inconsistent data caused by poor cell adhesion.
Breaking Down the Features of BD Purecoat Amine Flask 175 Cs50 356728
Specifications
The BD Purecoat Amine Flask 175 Cs50 356728 features a 175 cm² growth area, providing ample space for cell proliferation. The flasks are supplied in a case of 50, ensuring an adequate supply for extensive research projects. These flasks are categorized under MICROBIOLOGY and have the Supplier Number: 356728.
The large surface area of 175 cm² is crucial for experiments requiring a high cell yield. The amine coating promotes superior cell adhesion, which is vital for cells that have difficulty attaching to standard tissue culture surfaces. The case of 50 is convenient for labs that perform regular cell culture work.
Performance & Functionality
The BD Purecoat Amine Flask 175 Cs50 356728 excels at providing a stable and consistent platform for cell culture. Cells adhere strongly to the amine-coated surface, promoting healthy growth and accurate experimental results. The flask’s design minimizes contamination risks, ensuring reliable performance.
A major strength is its ability to support the growth of adhesion-sensitive cells, resulting in more reliable data. A minor area for improvement could be a slightly modified neck design for even easier media access. Overall, it exceeds my expectations.
Design & Ergonomics
The BD Purecoat Amine Flask 175 Cs50 356728 is made from high-quality, transparent plastic, allowing for easy visualization of cells. The weight is balanced and comfortable to handle during routine laboratory procedures. Its rectangular shape provides stability on the benchtop and in incubators.
The flasks are user-friendly, requiring no special skills or equipment for handling. The standard flask design ensures a smooth transition for researchers accustomed to typical cell culture protocols. The flasks are well-labeled and easy to identify.
Durability & Maintenance
With proper care and handling, the BD Purecoat Amine Flask 175 Cs50 356728 is designed to last for the duration of its intended use. The high-quality plastic material is resistant to cracking and warping under normal laboratory conditions. The amine coating is robust and remains effective after multiple uses.
Maintaining the flasks involves standard cell culture cleaning procedures, including rinsing with sterile water and sterilization via autoclaving. The amine coating is designed to withstand these processes without degradation, ensuring long-term usability. Regular inspection for cracks or damage is recommended to maintain optimal performance.
Accessories and Customization Options
The BD Purecoat Amine Flask 175 Cs50 356728 does not come with additional accessories. However, it’s fully compatible with standard cell culture media, supplements, and equipment. There are no specific customization options for the flask itself, as its primary function is to provide a consistent and reliable cell adhesion surface.
The flasks are compatible with various cell scrapers, aspirators, and other standard cell culture tools. The universal design allows seamless integration into existing laboratory setups. Researchers can easily adapt their existing protocols to utilize these flasks without significant modifications.
Pros and Cons of BD Purecoat Amine Flask 175 Cs50 356728
Pros
- Significantly enhanced cell adhesion compared to standard TC-treated flasks.
- Consistent and reliable performance across multiple experiments.
- User-friendly design integrates seamlessly into existing cell culture workflows.
- High-quality materials ensure durability and minimize contamination risks.
- Suitable for a wide range of cell types, including adhesion-sensitive cells like primary neurons.
Cons
- High price point may be a barrier for some labs.
- No included accessories (e.g., specialized caps or filters).
Who Should Buy BD Purecoat Amine Flask 175 Cs50 356728?
Perfect for: Researchers working with adhesion-sensitive cells, laboratories needing consistent and reliable cell culture performance, and scientists conducting high-throughput experiments. Anyone culturing primary cells or cell lines that are notoriously difficult to adhere will significantly benefit from this product.
Who should skip this product? Labs on a very tight budget or those primarily working with readily adherent cell lines might not need the enhanced adhesion properties offered by these flasks. Standard TC-treated flasks could be sufficient for their needs.
Must-have accessories or modifications? Standard cell culture media and supplements are essential. Cell scrapers and aspirators are also highly recommended for efficient cell harvesting.
Conclusion on BD Purecoat Amine Flask 175 Cs50 356728
The BD Purecoat Amine Flask 175 Cs50 356728 is a high-quality, reliable cell culture flask that delivers exceptional cell adhesion and consistent performance. While the price is substantial, the benefits of improved cell growth and reduced experimental variability justify the investment, especially for researchers working with challenging cell types. These flasks significantly improve the reliability of cell-based assays.
The value proposition is clear: increased data accuracy, reduced waste, and enhanced experimental success. The consistent cell adhesion alone is worth the price for specific applications. It allows researchers to focus on the experiment, not the cell culture.
I would absolutely recommend the BD Purecoat Amine Flask 175 Cs50 356728 to any researcher seeking to improve their cell culture results. If you are struggling with inconsistent cell adhesion or need a reliable platform for challenging cell types, this product is a worthwhile investment. Give your cells the best possible start and unlock new levels of experimental success with these flasks!