Let’s Dive into Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350
The Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350 is a high-throughput polymerase chain reaction (PCR) plate designed for efficient and reliable amplification of DNA samples. Manufactured by Eppendorf, a trusted name in laboratory equipment, this plate boasts 384 wells for maximizing sample processing. The blue color aids in easy identification and tracking in automated systems, while the pack of 10 ensures a ready supply for demanding laboratory workflows.
My work in high-throughput screening often involves amplifying hundreds of DNA samples, a task that was becoming a bottleneck with traditional 96-well plates. I needed a solution that would increase my throughput without sacrificing accuracy or reliability. That’s when I started looking into 384-well PCR plates.
Upon unboxing, the plates feel sturdy and well-constructed, exhibiting the quality I’ve come to expect from Eppendorf. The blue hue is consistent across all ten plates, and the individual wells are uniformly shaped and spaced. Initial handling suggests a plate designed for robotic handling, reducing the risk of spillage or cross-contamination.
I considered plates from other manufacturers like Thermo Fisher and Bio-Rad, but ultimately chose the Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350 for its reputation for consistent performance and compatibility with our existing PCR equipment. The well volume and plate dimensions were also a perfect fit for our robotic liquid handling system, a crucial factor in our decision. I was excited to see if these plates could truly streamline my workflow and increase our lab’s efficiency.
Real-World Testing: Putting Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350 to the Test
First Use Experience
I tested the Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350 in our molecular biology lab, using it for a large-scale genotyping project. We used a robotic liquid handler to load the plates with DNA samples and PCR reagents. The plate’s rigid construction prevented warping and ensured consistent liquid transfer, reducing pipetting errors.
The plates performed flawlessly under varying thermal cycling conditions, from standard PCR to fast cycling protocols. We also ran some tests under higher humidity to simulate real-world conditions in our lab during summer, and the plates maintained their structural integrity without any condensation issues within the wells. The plate was easy to handle when removing it from the thermocycler.
No significant issues arose during the first use, although I did notice that careful attention was needed when sealing the plates to prevent evaporation. The plate sealing film needed to be applied evenly and firmly to guarantee optimal results.
Extended Use & Reliability
After using the Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350 for several months, I’ve observed that the plates hold up remarkably well under constant use. Even after multiple cycles of thermal stressing, the plates show no signs of deformation or cracking. The blue dye doesn’t leach into the samples, which is a major concern with lower-quality plates.
The plates exhibit excellent durability and resistance to common laboratory solvents like ethanol and isopropanol used for cleaning. Maintaining the plates is simple, requiring only a quick wipe-down with a lint-free cloth. Compared to cheaper alternatives I’ve used in the past, these Eppendorf plates deliver far superior performance and reliability, justifying the higher price point.
Breaking Down the Features of Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350
Specifications
The Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350 is designed for high-throughput PCR applications. It features 384 wells, maximizing sample processing capacity. The Eppendorf plate is blue in color for easy visual identification. It comes in a pack of 10 plates.
The Eppendorf Pcr Plate is manufactured from virgin polypropylene, known for its low binding characteristics, preventing sample loss. The uniform well volume ensures consistent reaction conditions across all wells. These specifications translate to accurate and reproducible results.
Performance & Functionality
The Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350 performs exceptionally well in its intended application. The rigid design facilitates easy handling and robotic automation. The uniform well shape and precise dimensions ensure efficient heat transfer and optimal PCR amplification.
While the plates themselves are excellent, their effective use requires a good quality plate sealer. Improper sealing leads to sample evaporation and inconsistent results. The plate meets and exceeds expectations for high-throughput PCR.
Design & Ergonomics
The Eppendorf Pcr Plate’s blue color provides a clear visual contrast, simplifying sample tracking. The rigid polypropylene construction minimizes warping and bending, enhancing usability in automated systems. It’s comfortable to handle and easily fits into standard 384-well thermocyclers. There is virtually no learning curve.
Durability & Maintenance
The Eppendorf Pcr Plate is designed for durability, withstanding repeated thermal cycles without degradation. Proper cleaning and storage are essential to maintain their integrity and prevent contamination. With proper care, these plates can last through numerous experiments.
Accessories and Customization Options
The Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350 does not come with any specific accessories besides the pack of 10 plates. However, it is compatible with a wide range of plate sealers and robotic liquid handling systems. Compatible sealing films from brands like Eppendorf, Thermo Fisher, and VWR can be used. Different sealing films will be required based on your exact experimental needs.
Pros and Cons of Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350
Pros
- High throughput: 384 wells significantly increase sample processing capacity.
- Consistent performance: Uniform well volume and high-quality materials ensure reliable PCR amplification.
- Durable construction: Rigid polypropylene withstands repeated thermal cycles.
- Robot-friendly: Designed for automated liquid handling systems, minimizing human error.
- Easy to identify: The blue color aids in visual tracking and reduces the risk of sample mix-ups.
Cons
- Relatively expensive: Higher price compared to some alternative brands.
- Requires careful sealing: Proper sealing is essential to prevent evaporation and ensure accurate results.
Who Should Buy Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350?
The Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350 is perfect for researchers, molecular biologists, and laboratory technicians involved in high-throughput PCR, genotyping, and sequencing. It is also well-suited for diagnostic labs needing reliable and efficient sample processing.
Anyone on a tight budget or who doesn’t require high-throughput should skip this product. A suitable high-quality plate sealer is a must-have accessory.
Conclusion on Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350
The Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350 is a top-tier product that delivers exceptional performance and reliability for high-throughput PCR applications. While the price is higher than some alternatives, the consistent results and durable construction justify the investment. I would personally recommend the Eppendorf Pcr Plate to any lab seeking to streamline their PCR workflow and increase their sample processing capacity. If you need reliable, high-throughput PCR, don’t hesitate to invest in the Eppendorf Pcr Plate 384wl Mb Bl Pk10 0030129350.