Let’s Dive into VWR Comb 20 Well 1.5MM Page E2110-20-1.5
The VWR Comb 20 Well 1.5MM Page E2110-20-1.5, manufactured by VWR, is a seemingly unassuming piece of lab equipment. However, for anyone involved in gel electrophoresis, this comb is an indispensable tool for creating the wells needed to load samples. Its design and materials directly impact the precision and efficiency of the entire process, and in scientific research, precision is paramount.
My journey with gel electrophoresis began years ago, analyzing soil DNA samples for bacterial diversity in remote Alaskan watersheds. Proper well formation is absolutely critical for accurate band resolution and downstream analysis. I needed a reliable comb that would produce consistently sized and shaped wells, minimizing sample spillage and maximizing resolution.
Upon unboxing the VWR Comb 20 Well 1.5MM Page E2110-20-1.5, the first thing I noticed was the rigidity of the polyethylene plastic. This contrasts sharply with some of the cheaper, more flexible combs I’ve used in the past. The molded tweezers and rubber tether were also immediate standouts, suggesting a thoughtful design focused on user convenience and cleanliness.
Compared to generic combs available online, the VWR Comb 20 Well 1.5MM Page E2110-20-1.5 promised a higher degree of precision and reduced particle generation. Having battled with poorly formed wells and contaminated gels in the past, the promise of cleaner, more consistent results was extremely appealing. I chose this comb specifically for its reputation for low particle generation and the convenience of the integrated tweezers.
My initial impression was one of cautious optimism. The price point seemed high, but the features and the reputation of VWR suggested that the investment might be worthwhile. I was excited to see if it would truly live up to the promise of cleaner, more consistent electrophoresis results.
Real-World Testing: Putting VWR Comb 20 Well 1.5MM Page E2110-20-1.5 to the Test
First Use Experience
I first tested the VWR Comb 20 Well 1.5MM Page E2110-20-1.5 in my university lab, preparing agarose gels for DNA fragment separation. This was during a particularly humid summer, a condition that can sometimes affect gel polymerization and well formation. The comb slid into the gel form smoothly and created wells with clean edges, and the integrated tweezers proved useful for gentle comb removal.
Despite the humidity, the wells formed perfectly without any distortion or leakage. The ease of use was immediately apparent, and the well spacing was ideal for my standard pipette tips. There were no noticeable issues or surprises; the comb performed exactly as advertised.
Extended Use & Reliability
After several weeks of consistent use, the VWR Comb 20 Well 1.5MM Page E2110-20-1.5 continues to perform admirably. The plastic has shown no signs of warping or cracking, even after repeated exposure to gel solutions and cleaning agents. The tethered lid remains securely attached, preventing contamination.
The comb is holding up exceptionally well, showing no signs of wear and tear. Cleaning is simple: a quick rinse with distilled water is typically sufficient to remove any residual gel. Compared to previous experiences with cheaper combs that would often warp or crack after a few uses, the VWR Comb 20 Well 1.5MM Page E2110-20-1.5 has significantly outperformed them in terms of durability and reliability.
Breaking Down the Features of VWR Comb 20 Well 1.5MM Page E2110-20-1.5
Specifications
The VWR Comb 20 Well 1.5MM Page E2110-20-1.5 is specifically designed for use with 1.5 mm thick agarose or polyacrylamide gels. It features 20 wells, allowing for the simultaneous analysis of multiple samples. The comb is constructed from polyethylene plastic, known for its stiffness and resistance to chemical degradation.
The deep-well geometry minimizes particle generation, which is crucial for preventing contamination and ensuring clear electrophoresis results. The non-toxic, antistatic tweezers integrated into one end facilitate safe and easy handling. The rubber tether and well ring secure the lid, preventing dust and debris from entering the wells.
Performance & Functionality
The VWR Comb 20 Well 1.5MM Page E2110-20-1.5 excels at its primary function: creating uniform and clean wells for gel electrophoresis. The 1.5 mm thickness provides sufficient well volume for most standard sample loading requirements. Its design minimizes distortion during gel polymerization, resulting in sharp and well-defined bands.
The comb’s strengths lie in its precision, durability, and ease of use. One minor area for potential improvement might be the addition of well markers to aid in sample identification. Overall, the VWR Comb 20 Well 1.5MM Page E2110-20-1.5 meets and exceeds expectations in terms of performance and functionality.
Design & Ergonomics
The build quality of the VWR Comb 20 Well 1.5MM Page E2110-20-1.5 is excellent, with a smooth, consistent finish and no sharp edges. The polyethylene plastic is lightweight yet robust, providing a comfortable grip. The integrated tweezers are a thoughtful addition that enhances user convenience and reduces the risk of contamination.
The design is highly user-friendly, requiring no specialized training or techniques. The comb slides smoothly into the gel form and is easily removed, creating consistent wells every time.
Durability & Maintenance
Given its construction from high-quality polyethylene plastic, the VWR Comb 20 Well 1.5MM Page E2110-20-1.5 is expected to last for years with proper care. The material is resistant to cracking, warping, and chemical degradation, ensuring long-term reliability.
Maintenance is straightforward; a simple rinse with distilled water or a mild detergent is typically sufficient. The comb can also be autoclaved for sterilization, further extending its lifespan.
Accessories and Customization Options
The VWR Comb 20 Well 1.5MM Page E2110-20-1.5 does not require any additional accessories for basic operation, as it includes the essential tethered lid and integrated tweezers. However, users may choose to purchase additional combs with different well configurations (e.g., different well widths or numbers of wells) to suit specific experimental needs.
The comb is compatible with standard gel electrophoresis equipment and reagents from various brands. There are no specific customization options available for this product, but its modular design allows it to seamlessly integrate into existing laboratory workflows.
Pros and Cons of VWR Comb 20 Well 1.5MM Page E2110-20-1.5
Pros
- Precise well formation: Ensures consistent and well-defined bands in gel electrophoresis.
- Durable construction: Made from high-quality polyethylene plastic for long-lasting performance.
- Integrated tweezers: Facilitates safe and easy handling, reducing contamination risks.
- Low particle generation: Minimizes interference with electrophoresis results, enhancing accuracy.
- Easy to clean and maintain: Simplifies laboratory workflows and ensures hygienic conditions.
Cons
- Relatively high price: May be a barrier for budget-constrained labs.
- Limited customization options: No adjustable well spacing or other advanced features.
Who Should Buy VWR Comb 20 Well 1.5MM Page E2110-20-1.5?
The VWR Comb 20 Well 1.5MM Page E2110-20-1.5 is perfect for researchers, lab technicians, and students involved in molecular biology, biochemistry, and genetics. It’s especially beneficial for those who require consistent and reliable gel electrophoresis results. Any laboratory performing routine DNA or protein separation will benefit from the precision and durability of this comb.
This product might not be the best choice for individuals or institutions on extremely tight budgets, as cheaper alternatives are available. However, the long-term reliability and performance advantages of the VWR Comb 20 Well 1.5MM Page E2110-20-1.5 often justify the initial investment. A gel electrophoresis apparatus and appropriate buffers and dyes are must-have accessories.
Conclusion on VWR Comb 20 Well 1.5MM Page E2110-20-1.5
The VWR Comb 20 Well 1.5MM Page E2110-20-1.5 is a well-designed and highly functional tool that delivers consistent and reliable results in gel electrophoresis. While the price may seem steep compared to generic options, the durability, precision, and thoughtful features (like the integrated tweezers) make it a worthwhile investment for any lab that values accuracy and efficiency.
The price is justified by the superior performance and long lifespan of the comb. I would personally recommend it to any researcher or lab technician seeking a reliable and user-friendly solution for creating high-quality wells for gel electrophoresis. If you’re tired of inconsistent results and flimsy combs, upgrade to the VWR Comb 20 Well 1.5MM Page E2110-20-1.5 and experience the difference!