Let’s Dive into Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes
In the world of scientific research and development, reliable equipment is non-negotiable. The Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes promise just that – a dependable tool for critical separation processes. Manufactured by Kimble Chase, these 30mL tubes are designed to withstand the rigors of high-speed centrifugation and elevated temperatures.
My journey with lab equipment began over a decade ago, initially testing materials for durability in extreme environments. This led me to needing consistently reliable and safe centrifuge tubes, as my work involved extracting and separating vital components from samples exposed to harsh conditions. The search for tubes able to endure high G-forces and thermal cycling became my quest.
Upon receiving the Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes, I was immediately struck by their robust construction. The borosilicate glass felt substantial, offering a reassurance of durability. Compared to cheaper, thinner glass tubes I’d used in the past, these exuded quality.
Previously, I’ve used Corning and Eppendorf centrifuge tubes. I chose the Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes for their high G-force rating (13,100 g), as well as their stated ability to withstand high temperatures. My initial excitement was palpable; if these tubes performed as claimed, they would be a game-changer.
Real-World Testing: Putting Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes to the Test
First Use Experience
My first use of these tubes involved separating soil samples collected from a high-altitude alpine environment. The tubes were loaded into a centrifuge, reaching speeds necessary to isolate specific microorganisms from the sediment. They handled the initial 10,000 g spin without any issues, inspiring confidence.
These tubes performed flawlessly across varying temperature conditions, from -20°C during sample preparation to 4°C within the centrifuge. Cleaning was surprisingly straightforward. There were no observable cracks or structural weaknesses apparent after the initial run.
The Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes required no learning curve. These are simple plain-top tubes, making them quick to prepare and load. A positive note was the ease with which I could visually inspect the pellet formation due to the clear glass.
Extended Use & Reliability
After several weeks of consistent use, involving various sample types and centrifugation protocols, the Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes have demonstrated exceptional reliability. I subject them to rigorous daily workloads, consistently reaching their maximum rated G-force. There were no failures to report.
There are minimal signs of wear and tear, beyond minor surface abrasions. The glass remains resilient, demonstrating the quality of the borosilicate material. This durability is a huge improvement compared to tubes that cracked under similar conditions in the past.
The tubes are easily cleaned with standard lab detergents. Autoclaving does not seem to affect their structural integrity. The low maintenance requirements contribute significantly to the overall efficiency of my workflow.
Comparing them to my previous experience with less expensive tubes, these Kimble/Kontes tubes outperform significantly in terms of durability and reliability. The consistent performance justifies the higher initial investment.
Breaking Down the Features of Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes
Specifications
- Volume: 30 mL. This capacity is ideal for a wide range of applications, balancing sample size with centrifuge rotor compatibility.
- O.D. x L: 24 x 106 mm. These dimensions are standard for 30mL centrifuge tubes, ensuring compatibility with common centrifuge rotors.
- Cap Size: Plain-Top (no cap included). The plain-top design provides versatility, allowing for various closure methods depending on the specific experimental needs.
- Kimble No.: 45500-30. This is the manufacturer’s part number for easy reordering and identification.
- Maximum RCF: 13,100 g. This high G-force rating is a key selling point, enabling efficient separation of even the most challenging samples.
- Material: Type 1, Class B, chemically strengthened borosilicate glass. This type of glass offers excellent chemical resistance, thermal stability, and mechanical strength.
- Temperature Resistance: Up to 300°C (572°F). This high-temperature resistance allows for autoclaving and other high-temperature applications.
These specifications are critical for reliable and reproducible results. The high G-force rating, in particular, allows for faster and more complete separations. This enhances the overall efficiency of my lab procedures.
Performance & Functionality
The Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes excel in their primary function: efficiently and reliably separating substances through centrifugation. The robust construction and high G-force rating ensure consistent performance.
The tubes are strong and dependable, able to withstand considerable stress during use. While caps must be purchased separately, this allows customization based on specific lab requirements. Overall, they meet my expectations for a high-quality centrifuge tube.
Design & Ergonomics
The Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes are constructed from high-quality borosilicate glass. The design is straightforward and functional, with a round-bottom shape to facilitate pellet formation. The tube is comfortable to handle, and its weight provides a sense of durability.
The plain-top design is exceptionally user-friendly. Minimal preparation is needed before use. The clarity of the glass enables quick and easy visualization of the sample and resulting pellet.
Durability & Maintenance
The chemically strengthened borosilicate glass ensures a long lifespan for these tubes. They have consistently withstood the stresses of repeated high-speed centrifugation. With proper handling and care, these tubes should last for years.
The tubes are easy to clean using standard laboratory detergents. Autoclaving for sterilization is a seamless and effective process, with no noticeable degradation of the material.
Accessories and Customization Options
As plain-top tubes, the primary accessory required is a suitable cap or closure. Screw-thread caps with PTFE-faced rubber liners can be purchased separately for a secure seal. Accessory rubber adapter sleeves can be utilized when spinning these tubes in a 50mL rotor cavity.
These tubes are compatible with a wide range of centrifuge rotors designed for 30mL tubes. Different cap options allow for varying degrees of sealing, depending on the experiment’s requirements. The versatility in accessory selection makes these tubes adaptable to diverse laboratory setups.
Pros and Cons of Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes
Pros
- High G-force rating (13,100 g) for efficient separation.
- Excellent chemical and thermal resistance due to borosilicate glass construction.
- Durable and long-lasting, reducing the need for frequent replacements.
- Easy to clean and sterilize via autoclaving.
- Versatile plain-top design, compatible with various closure methods.
Cons
- Relatively high price compared to standard centrifuge tubes.
- Caps are not included and must be purchased separately.
Who Should Buy Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes?
These tubes are perfect for researchers, scientists, and laboratory technicians working with high-speed centrifugation and requiring robust, reliable tubes. They are particularly beneficial in fields such as microbiology, biochemistry, and environmental science.
These tubes may not be the best choice for budget-conscious labs that only require low-speed centrifugation. Also, labs that need pre-sterilized and pre-capped tubes might find these less convenient.
Labs requiring a secure seal will definitely need to purchase compatible caps. Rubber adapter sleeves are also required when using these tubes in a 50mL rotor cavity.
Conclusion on Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes
The Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes are a worthwhile investment for any lab that prioritizes reliability and durability. The high G-force rating and excellent material properties justify the price for demanding applications.
While the initial cost may be higher, the longevity and consistent performance of these tubes make them a cost-effective choice in the long run. I wholeheartedly recommend these tubes to anyone seeking a dependable solution for high-speed centrifugation.
If you need centrifuge tubes that can handle the pressure, look no further than the Kimble/Kontes High Strength Round-Bottom Centrifuge Tubes, Kimble Chase 45500-30 Plain-Top Tubes. You will not be disappointed.